ULTRASTRUCTURAL CHARACTERIZATION OF ADRENOCORTICOTROPE HORMONE (ACTH) IMMUNOREACTIVE FIBERS IN THE MESENCEPHALIC CENTRAL GREY SUBSTANCE OF THE RAT
ULTRASTRUCTURAL CHARACTERIZATION OF ADRENOCORTICOTROPE HORMONE (ACTH) IMMUNOREACTIVE FIBERS IN THE MESENCEPHALIC CENTRAL GREY SUBSTANCE OF THE RAT
复制标题
DOI：
10.1111/j.1460-9568.1989.tb00372.x
复制
发表时间：
1989-11-01
影响因子：
3.4
通讯作者：
NIEUWENHUYS, R
中科院分区：
文献类型：
--
作者：
BUMA, P;VEENING, J;NIEUWENHUYS, R
研究了雄性Wistar大鼠中脑中央灰色物质（MCG）中促肾上腺皮质激素（ACTH）免疫反应纤维的精细结构定位。光镜下，在背侧、腹侧、室周位置的整个MCG中发现了曲张的ACTH免疫反应纤维。电镜下，在轴突静脉曲张的电子致密分泌颗粒的直接附近，以及在其他细胞器（如电子透明突触囊泡、线粒体和微管）周围，尽管程度较低，但免疫反应性最为突出。通过连续切片分析，发现两种类型的ACTH免疫反应性静脉曲张。第一种是大的，包含许多小的电子透明突触囊泡，它们位于形态明确的突触接触附近。在这种类型的静脉曲张中，很少有大的致密核分泌颗粒。免疫反应性低或无，特别是在活性区附近。第二种是强烈的免疫反应。它总是含有许多大的，致密的核心分泌颗粒；电子发光囊泡是罕见的。这种类型的小静脉曲张从不发生突触接触，但少数较大的静脉曲张与MCG细胞的树突有突触接触。
The fine structural localization of fibres immunoreactive for the adrenocorticotrope hormone (ACTH) was studied in the mesencephalic central grey substance (MCG) of the male Wistar rat. Light microscopically, varicose ACTH‐immunoreactive fibres were found throughout the MCG in a dorsal, lateral and ventral, periventricular position. Electron microscopically, the immunoreactivity was most prominent in the direct vicinity of electron‐dense secretory granules in axonal varicosities, and, although to a lower degree, around other cytoplasmic organelles such as electron‐lucent synaptic vesicles, mitochondria and microtubules. With serial section analysis two types of ACTH‐immunoreactive varicosity were discerned. The first type is large, contains many, small electron‐lucent synaptic vesicles, that are located in the vicinity of a morphologically well‐defined synaptic contact. In this type of varicosity, large dense‐core secretory granules are scarce. Immunoreactivity is low or absent, particularly near the active zone. The second type is strongly immunoreactive. It always contains many large, dense‐core secretory granules; electron‐lucent vesicles are rare. The smaller varicosities of this type never make synaptic contacts, but a few of the larger varicosities have synaptic contacts with dendrites of MCG cells.