Application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes.
Application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes.
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DOI：
10.1111/j.1751-7915.2008.00083.x
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发表时间：
2009-03
影响因子：
5.7
通讯作者：
Parales RE
中科院分区：
文献类型：
--
作者：
Ju KS;Parales RE
氯硝基苯作为工业化学品和药品生产原料的广泛应用，造成了这些有毒化合物对环境的广泛污染，对人类和野生动物的健康构成重大风险。虽然生物处理通常是一种有吸引力的修复解决方案，但由于能够有效矿化这些化合物的菌株数量很少，并且它们只能降解选定的异构体，因此氯硝基苯的有效性受到限制。为了满足这一需求，我们创造了具有新型降解途径的工程菌株，减少了将氯硝基苯转化为中心代谢化合物所需的总步骤。我们检测了来自Acidovorax sp.菌株JS42的2 -硝基甲苯2,3 -双加氧酶、来自Comamonas sp.菌株JS765的硝基苯1,2 -双加氧酶（NBDO）以及NBDO活性位点突变体从氯硝基苯生成氯邻苯二酚的能力，并确定了效率最高的酶。将野生型NBDO和F293Q变体引入到Ralstonia sp.菌株JS705（一种携带氯儿茶酚代谢修饰的邻位途径的菌株）中，结果菌株能够在所有三种氯硝基苯异构体上持续生长，而无需添加共底物或共诱导物。这些第一代工程菌株证明了硝基芳烃双加氧酶在扩大细菌代谢能力方面的效用，并为改进氯硝基苯污染场所的生物处理提供了新的选择。
Widespread application of chloronitrobenzenes as feedstocks for the production of industrial chemicals and pharmaceuticals has resulted in extensive environmental contamination with these toxic compounds, where they pose significant risks to the health of humans and wildlife. While biotreatment in general is an attractive solution for remediation, its effectiveness is limited with chloronitrobenzenes due to the small number of strains that can effectively mineralize these compounds and their ability to degrade only select isomers. To address this need, we created engineered strains with a novel degradation pathway that reduces the total number of steps required to convert chloronitrobenzenes into compounds of central metabolism. We examined the ability of 2‐nitrotoluene 2,3‐dioxygenase from Acidovorax sp. strain JS42, nitrobenzene 1,2‐dioxygenase (NBDO) from Comamonas sp. strain JS765, as well as active‐site mutants of NBDO to generate chlorocatechols from chloronitrobenzenes, and identified the most efficient enzymes. Introduction of the wild‐type NBDO and the F293Q variant into Ralstonia sp. strain JS705, a strain carrying the modified ortho pathway for chlorocatechol metabolism, resulted in bacterial strains that were able to sustainably grow on all three chloronitrobenzene isomers without addition of co‐substrates or co‐inducers. These first‐generation engineered strains demonstrate the utility of nitroarene dioxygenases in expanding the metabolic capabilities of bacteria and provide new options for improved biotreatment of chloronitrobenzene‐contaminated sites.
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影响因子：
4.4
作者：
Ju, KS;Parales, RE
通讯作者：
Parales, RE
DOI：
10.1016/s0021-9673(01)93159-0
发表时间：
1990-09-28
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
FELTES, J;LEVSEN, K;SPIEKERMANN, M
通讯作者：
SPIEKERMANN, M
影响因子：
4.4
作者：
Johnson, GR;Smets, BF;Spain, JC
通讯作者：
Spain, JC
影响因子：
3.8
作者：
LIVINGSTON, AG
通讯作者：
LIVINGSTON, AG
影响因子：
2.9
作者：
GIBSON, DT;KOCH, JR;KALLIO, RE
通讯作者：
KALLIO, RE