Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies
Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies
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DOI：
10.1016/j.msec.2018.10.048
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发表时间：
2019-01-01
影响因子：
7.9
通讯作者：
Kellomaki, Minna
中科院分区：
文献类型：
--
作者：
Karvinen, Jennika;Ihalainen, Teemu O.;Kellomaki, Minna
微观结构对水凝胶性能的控制起着至关重要的作用。当细胞或药物被包裹在水凝胶中时，这也是一个重要的因素。在这项工作中，首次采用基于流变学和扩散（光漂白后荧光恢复，FRAP）的方法对腙交联透明质酸、海藻酸盐和结冷胶基水凝胶的微观结构进行了全面的评价。揭示了凝胶参数对水凝胶粘弹性和扩散性能的影响，以及对水凝胶结构参数（网目尺寸、相邻交联聚合物链的平均分子量、交联密度）的影响。结果进一步表明，当使用较大的葡聚糖尺寸时，扩散率下降，这相当于用流变学方法评估的水凝胶的粒径（15 nm至47 nm）。这个网孔大小范围允许小分子的运输，也允许多肽和大多数蛋白质的运输。存储模量与结构参数之间也存在相关性。总的来说，通过改变凝胶参数，腙交联提供了一种简单的方法来生产具有可变微观结构的多糖基水凝胶。
Microstructure plays an essential role in the control of hydrogel properties. It is also an important factor when cells or drugs are encapsulated inside the hydrogel. In this work, the microstructures of hydrazone crosslinked hyaluronan-, alginate- and gellan gum-based hydrogels were evaluated thoroughly for the first time by using rheology- and diffusion (fluorescence recovery after photobleaching, FRAP)-based methods. The effect of gel parameters on the viscoelastic and diffusion properties of hydrogels, and further on their structural parameters (mesh size, average molecular weight of the polymer chain between neighboring crosslinks, crosslinking density) are shown. Results further show that diffusivity decreased when larger dextran sizes were used, which were equivalent to the mesh sizes of hydrogels (15 nm to 47 nm) evaluated by the rheological method. This mesh size range allows the transportation of smaller molecules, but also peptides and most of the proteins. A correlation between the storage modulus and the structural parameters was also shown. Overall, hydrazone crosslinking offers an easy way to produce polysaccharide-based hydrogels with variable microstructures by altering the gel parameters.