Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field.
Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field.
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DOI：
10.1038/s41467-021-24790-6
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发表时间：
2021-07-28
影响因子：
16.6
通讯作者：
Crisanti A
中科院分区：
文献类型：
--
作者：
Hammond A;Pollegioni P;Persampieri T;North A;Minuz R;Trusso A;Bucci A;Kyrou K;Morianou I;Simoni A;Nolan T;Müller R;Crisanti A
基于CRISPR的基因驱动器靶向疟疾媒介冈比亚按蚊中的doublex基因，有效地抑制了在小型实验室笼子里饲养的蚊子种群的繁殖能力。为了弥合实验室和田间之间的差距，这种基因驱动技术必须受到载体生态学的挑战。在这里，我们报告的抑制活性的基因驱动年龄结构的AN。冈比亚种群生活在大型室内笼子里，可以进行复杂的进食和繁殖行为。基因驱动元件在种群中迅速传播，在一年内完全抑制种群，而不选择对基因驱动的抗性。近似贝叶斯计算允许追溯推断的生活史参数从大笼子和更准确的预测基因驱动的行为下更生态相关的设置。为侵入性技术生成连接实验室和现场研究的数据是一项挑战。我们的研究代表了基于基因驱动的媒介控制工具的逐步和健全的发展模式。基因驱动种群动力学的实验分析大多局限于小型笼试验。为了填补实验室研究和田间研究之间的差距，作者在这里使用大型室内笼子，在没有出现抗性等位基因的情况下观察种群抑制
CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles gambiae effectively suppressed the reproductive capability of mosquito populations reared in small laboratory cages. To bridge the gap between laboratory and the field, this gene-drive technology must be challenged with vector ecology. Here we report the suppressive activity of the gene-drive in age-structured An. gambiae populations in large indoor cages that permit complex feeding and reproductive behaviours. The gene-drive element spreads rapidly through the populations, fully supresses the population within one year and without selecting for resistance to the gene drive. Approximate Bayesian computation allowed retrospective inference of life-history parameters from the large cages and a more accurate prediction of gene-drive behaviour under more ecologically-relevant settings. Generating data to bridge laboratory and field studies for invasive technologies is challenging. Our study represents a paradigm for the stepwise and sound development of vector control tools based on gene-drive. Experimental analysis of gene drive population dynamics has mostly been limited to small cage trials. Here the authors, to fill the gap between lab based studies and field studies, use large indoor cages and see population suppression without the emergence of resistant alleles
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影响因子：
5.4
作者：
North, Ace R.;Burt, Austin;Godfray, H. Charles J.
通讯作者：
Godfray, H. Charles J.
DOI：
10.1073/pnas.1713825115
发表时间：
2018-06-12
影响因子：
11.1
作者：
KaramiNejadRanjbar, Mohammad;Eckermann, Kolja N.;Wimmer, Ernst A.
通讯作者：
Wimmer, Ernst A.
影响因子：
3.3
作者：
James, Stephanie;Collins, Frank H.;Toure, Yeya T.
通讯作者：
Toure, Yeya T.
影响因子：
2.1
作者：
James, Stephanie L.;Marshall, John M.;Nolan, Tony
通讯作者：
Nolan, Tony
影响因子：
46.9
作者：
Kyrou K;Hammond AM;Galizi R;Kranjc N;Burt A;Beaghton AK;Nolan T;Crisanti A
通讯作者：
Crisanti A