The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice
The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice
复制标题
水稻幼苗形成需要异源三聚体 G 蛋白 β 亚基 RGB1
DOI：
10.1186/s12284-019-0313-y
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发表时间：
2019-07-18
期刊：
影响因子：
5.5
通讯作者：
Liang, Guohua
中科院分区：
文献类型：
--
作者：
Gao, Yun;Gu, Houwen;Liang, Guohua
背景：异源三聚体G蛋白β亚基RGB 1在植物生长发育中起重要作用。结果：利用CRISPR/Cas9系统分离到rgb 1突变体rgb 1 -1（+1bp）、rgb 1 -2（-1bp）和rgb 1 -3（-11bp），它们在萌发后1d被捕获，并最终表现出幼苗致死性。RGB 1幼苗和WT的胚胎在早期postgermination的动态解剖特征，并根据TUNEL检测表明，抑制生长的RGB 1突变体是由细胞死亡。rgb 1突变体除了限制芽和根的发育外，胚茎-根轴的发育也受到抑制。RGB 1主要在胚的根表皮和维管组织中表达。此外，转录谱分析显示，在幼苗发育过程中，与野生型相比，rgb 1突变体中大量的生长素、细胞分裂素和油菜素类固醇诱导基因的表达上调或下调。总的来说，rgb 1突变体为利用G蛋白研究水稻萌发后早期成苗的分子机制提供了理想的材料。异源三聚体G蛋白β亚基RGB 1是促进水稻萌发后早期幼苗发育的关键因子。
Background: The heterotrimeric G protein beta subunit RGB1 plays an important role in plant growth and development. However, the molecular mechanisms underlying the regulation of rice growth by RGB1 remain elusive.Results: Here, the rgb1 mutants rgb1-1 (+1bp), rgb1-2 (-1bp), and rgb1-3 (-11bp) were isolated using the CRISPR/Cas9 system, and they were arrested at 1day after germination and ultimately exhibited seedling lethality. The dynamic anatomical characteristics of the embryos of the rgb1 seedlings and WT during early postgermination and according to TUNEL assays showed that the suppressed growth of the rgb1 mutants was caused by cell death. In addition to the limited shoot and root development, the development of the embryo shoot-root axis was suppressed in the rgb1 mutants. RGB1 was expressed mainly in the root epidermal and vascular tissues of the embryo. Moreover, transcript profiling analysis revealed that the expression of a large number of auxin-, cytokinin-, and brassinosteroid-inducible genes was upregulated or downregulated in the rgb1 mutant compared to the wild type during seedling development.Conclusions: Overall, the rgb1 mutants provide an ideal material for exploring the molecular mechanism underlying rice seedling formation during early postgermination development by G proteins.Significance statement The heterotrimeric G protein beta subunit RGB1 acts as a crucial factor in promoting early postgermination seedling development in rice.