Effects of acupuncture on declined cerebral blood flow, impaired mitochondrial respiratory function and oxidative stress in multi-infarct dementia rats
Effects of acupuncture on declined cerebral blood flow, impaired mitochondrial respiratory function and oxidative stress in multi-infarct dementia rats
复制标题
针刺对多发梗塞性痴呆大鼠脑血流量下降、线粒体呼吸功能受损和氧化应激的影响
DOI：
10.1016/j.neuint.2013.12.004
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发表时间：
2014-01-01
影响因子：
4.2
通讯作者：
Yu, Jianchun
中科院分区：
文献类型：
--
作者：
Zhang, Xuezhu;Wu, Bangqi;Yu, Jianchun
慢性低灌注导致的脑能量代谢紊乱和氧化应激被认为是痴呆发病机制中的主要危险因素。在以往的研究中，我们已经证明针刺治疗可改善血管性痴呆（VaD）患者和多发性脑梗死痴呆（MID）大鼠的认知功能。针刺疗法还能提高大脑中糖代谢酶的活性。但是，针刺治疗是否通过增强葡萄糖代谢酶的活性和保护线粒体功能来弥补脑缺血后的神经元能量缺失，以及针刺的神经保护作用是否与线粒体抗氧化防御系统的激活有关，尚不清楚。因此，本研究探讨了针刺疗法对MID大鼠认知功能、脑血流量（CBF）、线粒体呼吸功能和脑内氧化应激的影响。结果表明，针刺治疗显著改善了MID大鼠的认知能力，并增加了其局部脑血流量。针刺提高了总超氧化物歧化酶（SOD）、铜锌超氧化物歧化酶（CuZnSOD）和锰超氧化物歧化酶（MnSOD）的活性，降低了丙二醛（MDA）和超氧阴离子的水平，调节了线粒体中还原型谷胱甘肽（GSH）与氧化型谷胱甘肽（GSSG）的比例，并提高了MID大鼠的呼吸控制指数（RCI）、磷氧比（P/O）以及线粒体呼吸酶的活性。这些结果表明，针刺治疗改善了MID大鼠的认知功能；这种改善可能是由于脑血流量增加，从而改善了缺血诱导的线粒体功能障碍以及脑内源性氧化应激系统。（C）2013爱思唯尔有限公司。保留所有权利。
Brain energy disorders and oxidative stress due to chronic hypoperfusion were considered to be the major risk factors in the pathogenesis of dementia. In previous studies, we have demonstrated that acupuncture treatment improved cognitive function of VaD patients and multi-infarct dementia (MID) rats. Acupuncture therapy also increased the activities of glycometabolic enzymes in the brain. But it is not clear whether acupuncture treatment compensates neuronal energy deficit after cerebral ischemic through enhancing the activities of glucose metabolic enzymes and preserving mitochondria] function, and whether acupuncture neuroprotective effect is associated with activations of mitochondrial antioxidative defense system. So, the effect of acupuncture therapy on cognitive function, cerebral blood flow (CBF), mitochondrial respiratory function and oxidative stress in the brain of MID rats was investigated in this study. The results showed that acupuncture treatment significantly improved cognitive abilities and increased regional CBF of MID rats. Acupuncture elevated the activities of total SOD, CuZnSOD and MnSOD, decreased the level of malondialdehyde (MDA) and superoxide anion, regulated the ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG) in mitochondria, and raised the level of the respiratory control index (RCI) and P/O ratio and the activities of mitochondrial respiratory enzymes of MID rats. These results indicated that acupuncture treatment improved cognitive function of MID rats; and this improvement might be due to increased CBF, which ameliorated mitochondrial dysfunction induced by ischemia and endogenous oxidative stress system of brain. (C) 2013 Elsevier Ltd. All rights reserved.