Monoclonal antibody A7 coupled to magnetic particles as a contrast enhancing agent for magnetic resonance imaging of human colorectal carcinoma
Monoclonal antibody A7 coupled to magnetic particles as a contrast enhancing agent for magnetic resonance imaging of human colorectal carcinoma
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DOI：
10.1007/s00262-005-0067-9
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发表时间：
2006-06-01
影响因子：
5.8
通讯作者：
Yamagishi，H
中科院分区：
文献类型：
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作者：
Otsuji，E;Kuriu，Y;Yamagishi，H
背景：局部复发是直肠癌最常见的复发方式，几乎总是致命的。局部复发的诊断困难是导致预后不良的重要原因。方法：将与人结直肠癌特异反应的单抗(Mab)A7偶联到铁磁性木素位点(FML)颗粒上，通过磁共振成像将直肠癌与其他组织区分开来。结果：A7-FML具有与单抗A7几乎相同的结合活性。与125I标记的正常小鼠Ig G-FML复合体相比，125I标记的A7-FML在移植瘤中的蓄积量显著增加(P<；0.05)。A7-FML从血液中迅速消失。与肿瘤相比，正常组织中125I标记的A7-FML积聚较少；这种积聚随时间呈线性下降。在磁共振成像中，注射A7-FML可降低肿瘤的信号强度。结论：A7-FML可作为结直肠癌皮下移植瘤的MR增强剂。
Background: Local recurrence, the most frequent pattern of recurrence of rectal carcinoma, is almost always fatal. The difficulty of diagnosing local recurrence contributes importantly to the poor prognosis.Methods: We coupled monoclonal antibody (Mab) A7, which reacts specifically with human colorectal carcinoma, to ferromagnetic lignosite (FML) particles to distinguish rectal carcinoma from other tissues by magnetic resonance (MR) imaging. We examined retention of immunoreactivity by the A7-FML complexes in vitro, and also their distribution in vivo according to radiolabeling and MR imaging when injected into nude mice bearing human colorectal carcinoma xenografts.Results: A7-FML retained binding activity nearly identical to that of Mab A7. Significantly more125I-labeled A7-FML accumulated in engrafted tumors than did125I-labeled normal mouse IgG-FML complexes (P<0.05). A7-FML disappeared rapidly from the blood. Normal tissues accumulated less125I-labeled A7-FML than tumors; this accumulation decreased linearly with time. In MR imaging, signal intensity was reduced in the tumor by the injection of A7-FML.Conclusions: A7-FML is potentially useful as a MR contrast enhancing agent for human colorectal carcinoma xenografts implanted subcutaneously.