Cardiovascular development in the zebrafish. I. Myocardial fate map and heart tube formation.
Cardiovascular development in the zebrafish. I. Myocardial fate map and heart tube formation.
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DOI：
10.1242/dev.119.1.31
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发表时间：
1993-09
期刊：
影响因子：
4.6
通讯作者：
D. Stainier;Robert K. K. Lee-Robert-K.-K.-Lee-2107716099;M. Fishman
中科院分区：
文献类型：
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作者：
D. Stainier;Robert K. K. Lee-Robert-K.-K.-Lee-2107716099;M. Fishman
我们用谱系示踪染料注射单个卵裂球，分析了斑马鱼胚胎中心脏祖细胞的来源，并用免疫组化染色的胚胎连续切片观察了斑马鱼心管的形成。在512细胞期(早期囊胚)，大多数心脏祖细胞位于从90度经度(未来的背轴和腹轴的中间)到180度经度(未来的腹轴)到270度经度的边缘地带。通过对位于90度(和270度)经度的心肌祖细胞的研究，我们发现，在早期囊胚中注射的单个细胞可以同时为心房和心室提供后代。注射到中囊胚内的细胞可以将后代贡献给心房或脑室，但不是两者都有。这一分析表明，至少对于这些心肌祖细胞来说，在中期胚泡中，心房和心室谱系是分开的。心前膜细胞在原肠发育过程中早期内折，并与其他早期内折的细胞一起转向动物极点。这些心源性细胞在8体节左右到达胚轴，在那里它们在中线两侧结合形成一对心肌小管原基。到21号体节时，原肌球蛋白免疫反应的心肌管相互靠近，一组独特的细胞--心内膜前体细胞--位于它们之间的中间。然后，心肌管融合，包围心内膜细胞，形成最终的心管。受精后22小时(26-体节体期)，心脏明显跳动。心肌肌球蛋白重链表达的区域化在这个阶段区分了心腔，尽管它们直到36小时才从形态上被描绘出来。这项工作表明，在早期囊胚中可以识别出心源性区域，而腔室限制似乎出现在中期囊胚中。此外，它还为单细胞水平的胚胎微扰以及斑马鱼心管形成的遗传分析提供了基础。
We have analyzed the origin of cardiac progenitors in the zebrafish embryo by injection of single blastomeres with a lineage tracer dye, and examined the formation of the zebrafish heart tube by serial sectioning of immunostained embryos. At the 512-cell stage (early blastula), most cardiac progenitors lie in a marginal zone that extends from 90 degrees longitude (midway between the future dorsal and ventral axis) through 180 degrees longitude (the future ventral axis) to 270 degrees longitude. By focusing on myocardial progenitors located at 90 degrees (and 270 degrees) longitude, we found that a single cell injected in the early blastula can contribute progeny to both the atrium and ventricle. A cell injected in the midblastula contributes progeny to either the atrium or ventricle, but not both. This analysis suggests that, at least for these myocardial progenitors, the atrial and ventricular lineages separate in the midblastula. Precardiac cells involute early during gastrulation and turn towards the animal pole with other early involuting cells. These cardiogenic cells reach the embryonic axis around the 8-somite stage, and there they coalesce to form a pair of myocardial tubular primordia on either side of the midline. By the 21-somite stage, the tropomyosin-immunoreactive myocardial tubes have moved closer to each other, and a distinct group of cells, the endocardial progenitor cells, sits medially between them. The myocardial tubes then fuse to enclose the endocardial cells and form the definitive heart tube. By 22 hours postfertilization (26-somite stage), the heart tube is clearly beating. The regionalization of cardiac myosin heavy chain expression distinguishes the cardiac chambers at this stage, although they are not morphologically delineated until 36 hours. This work shows that cardiogenic regions can be identified in the early blastula, and that chamber restriction seems to arise in the midblastula. Additionally, it provides the basis for embryological perturbation at the single cell level, as well as for the genetic analysis of heart tube formation in the zebrafish.