Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming.
Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming.
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DOI：
10.1111/nyas.12989
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发表时间：
2016-04
影响因子：
5.2
通讯作者：
Moore K
中科院分区：
文献类型：
--
作者：
Daniel MG;Lemischka IR;Moore K
尽管干细胞治疗和再生医学的所有范例都是从造血干细胞（HSC）的研究中出现的，但我们无法重新产生这些细胞或在体外扩增它们。获得这些细胞的最初努力始于使用胚胎干细胞（ESC）和诱导多能干细胞（iPSC）技术，但这些策略尚未产生功能齐全的细胞。随后，最近的方法涉及转录因子（TF）过表达以重编程PSC和各种体细胞。Shinya Yamanaka仅用四个TF诱导多能性告知了我们转换细胞命运的能力，并证明了利用终末分化细胞产生具有多谱系潜力的细胞的可行性。在这篇综述中，我们讨论了最近的努力，使用TF为基础的重编程策略，将几种细胞类型转化为HSC。
Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), our inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Shinya Yamanaka informs our ability to convert cell fates, and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF-based reprogramming strategies to convert several cell types into HSCs.