PLATELET-DERIVED GROWTH-FACTOR IN ASTHMA
PLATELET-DERIVED GROWTH-FACTOR IN ASTHMA
复制标题
DOI：
10.1111/j.1398-9995.1995.tb02493.x
复制
发表时间：
1995-11-01
期刊：
影响因子：
12.4
通讯作者：
BOUSQUET, J
中科院分区：
文献类型：
--
作者：
CHANEZ, P;VIGNOLA, M;BOUSQUET, J
血小板衍生生长因子（PDGF）控制细胞生长、迁移和分化。它由多种细胞类型分泌，包括巨噬细胞，并参与组织修复和上皮再生。因此PDGF可能参与哮喘气道重塑。本研究比较了哮喘患者和对照组支气管活检中PDGF及其受体（R α和R β）的免疫反应性以及支气管肺泡灌洗液中PDGF的水平。采用柔性支气管镜对11例哮喘患者和11例对照组进行支气管亚段活检。用单克隆抗体对PDGF AA、BE和PDGF受体Rr、RP进行研究，免疫过氧化物酶染色。研究了PDGFs及其受体在上皮和粘膜下层呈阳性染色的百分比。采用特异性ELISA法测定18例哮喘患者和10例对照患者BAL液中PDGF AA、AB、BB的含量。在活组织检查中，哮喘患者和对照组在PDGF AA、BB、PDGF-R α和R β方面没有显著差异（Fisher的精确检验和Bonferroni的校正）。此外，哮喘组和对照组的PDGF、AA、AB和BB水平相似。本研究不支持PDGF在哮喘修复过程中的作用。
Platelet-derived growth factor (PDGF) controls cellular growth, migration, and differentiation. It is secreted by various cell types, including macrophages, and participates in tissue repair and epithelial regeneration. PDGF may therefore be involved in airway remodeling in asthma. This study compared the immunoreactivity of PDGF and its receptors (R alpha and R beta) in bronchial biopsies and the levels of PDGF in bronchoalveolar lavage (BAL) fluid of asthmatics and control subjects. Bronchial biopsies were done in a subsegmental bronchus of 11 asthmatics and 11 control subjects by flexible bronchoscope. PDGF AA and BE, and PDGF receptors Rr and RP were studied with monoclonal antibodies and revealed by immunoperoxidase staining. The percentage of subjects presenting positive staining with PDGFs and its receptors was studied in the epithelium and submucosa. PDGF AA, AB, and BB were measured in BAL fluid of 18 asthmatics and 10 controls by specific ELISA. In biopsies, there was no significant difference between asthmatics and controls for PDGF AA, BB, PDGF-R alpha and R beta (Fisher's exact test and Bonferroni's correction). Moreover, the levels of PDGF, AA, AB, and BB were similar in asthmatics and controls. This study does not support a role for PDGF in the repair processes of asthma.