The salicylate hydroxylation assay to measure hydroxyl free radicals induced by local application of glutamate in vivo or induced by the Fenton reaction in vitro
The salicylate hydroxylation assay to measure hydroxyl free radicals induced by local application of glutamate in vivo or induced by the Fenton reaction in vitro
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DOI：
10.1016/s1385-299x(00)00014-3
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发表时间：
2000-04-01
期刊：
影响因子：
--
通讯作者：
Ferger, B
中科院分区：
文献类型：
--
作者：
Teismann, P;Ferger, B
在活体内直接测量羟基自由基是极其困难的。因此，用水杨酸盐(2-羟基苯甲酸酯)间接测定羟基自由基的方法被广泛接受。用谷氨酸进行反向微渗析后，水杨酸的羟基化加合物--2，3-二羟基苯甲酸--产生的羟基自由基呈剂量依赖关系。局部刺激羟基自由基的形成似乎适合于研究潜在的神经保护药物的清除自由基的特性。使用芬顿反应的体外实验可能是一个有用的工具，以评估一种物质是否能够在无细胞环境中作为自由基清除剂，这是一种在进行体内实验之前易于操作和简单的筛选方法。在目前的研究中，我们提出了一种体内方法，使用局部应用谷氨酸到纹状体，并在体外筛选使用芬顿反应，以诱导羟基自由基的形成。它的主要目标是可靠地测量羟基自由基，它是生物和医学中最活跃的氧自由基。(C)2000 Elsevier Science B.V.保留所有权利。
The direct measurement of hydroxyl radicals in vivo is extremely difficult. Therefore, the indirect determination of hydroxyl radicals using salicylate (2-hydroxybenzoate) is widely accepted. Reverse microdialysis with glutamate led to a dose-dependent production of hydroxyl free radicals indicated by the hydroxylation adduct of salicylate, namely 2,3-dihydroxybenzoic acid. The local stimulation of hydroxyl free radical formation seems to be suitable to investigate a radical-scavenging property of potential neuroprotective drugs. In vitro experiments using the Fenton reaction may be a helpful tool to assess whether or not a substance is able to act as a radical scavenger in a cell free environment, which is easy to handle and a simple screening method before in vivo experiments were performed. In the present study we present an in vivo approach using local application of glutamate into the striatum and an in vitro screening using the Fenton reaction to induce hydroxyl radical formation. The main goal is to reliable measure hydroxyl free radicals, which are the most reactive oxygen radicals in biology and medicine. (C) 2000 Elsevier Science B.V. All rights reserved.