Large-scale gene expression reveals different adaptations of Hyalopterus persikonus to winter and summer host plants
Large-scale gene expression reveals different adaptations of Hyalopterus persikonus to winter and summer host plants
复制标题
大规模基因表达揭示了Hyalopterus persikonus对冬季和夏季寄主植物的不同适应
DOI：
10.1111/1744-7917.12336
复制
发表时间：
2017-06-01
期刊：
影响因子：
4
通讯作者：
Cui, Feng
中科院分区：
文献类型：
--
作者：
Cui, Na;Yang, Peng-Cheng;Cui, Feng
寄主更替是指具有亲缘关系的寄主植物之间的季节性转移，对蚜虫超家族的多样性和成功具有重要影响。然而，潜在的分子机制仍不清楚。本研究通过对波斯粉蚜Hyalopterus periskonus在冬、夏寄主植物上的基因表达进行大规模分析，探讨了其寄主转换的分子机制。四倍多的unigenes的粉蚜显着上调夏季主机芦苇比冬季主机蔷薇科植物。为了筛选与寄主更替相关的候选基因，对H.将persikonus与豌豆无芒管虫唾液腺表达基因和分泌组进行比较。与核糖体和氧化磷酸化有关的基因以及具有血红素铜末端氧化酶活性、水解酶活性和核糖体结合等分子功能的基因在H. persikonus在夏季主机。推测的分泌蛋白，如解毒酶（羧酸酯酶和细胞色素P450），抗氧化酶（过氧化物酶和超氧化物歧化酶），谷胱甘肽过氧化物酶，葡萄糖脱氢酶，血管紧张素转换酶，钙粘蛋白和钙网蛋白，在H.在夏季寄主上的蚜虫中，一种SCP GAPR-1样家族蛋白和一种唾液鞘蛋白在夏季寄主上的蚜虫中高表达。这些结果揭示了蚜虫寄主利用和季节适应的表型可塑性。
Host alternation, an obligatory seasonal shifting between host plants of distant genetic relationship, has had significant consequences for the diversification and success of the superfamily of aphids. However, the underlying molecular mechanism remains unclear. In this study, the molecular mechanism of host alternation was explored through a large-scale gene expression analysis of the mealy aphid Hyalopterus persikonus on winter and summer host plants. More than four times as many unigenes of the mealy aphid were significantly upregulated on summer host Phragmites australis than on winter host Rosaceae plants. In order to identify gene candidates related to host alternation, the differentially expressed unigenes of H. persikonus were compared to salivary gland expressed genes and secretome of Acyrthosiphon pisum. Genes involved in ribosome and oxidative phosphorylation and with molecular functions of heme-copper terminal oxidase activity, hydrolase activity and ribosome binding were potentially upregulated in salivary glands of H. persikonus on the summer host. Putative secretory proteins, such as detoxification enzymes (carboxylesterases and cytochrome P450s), antioxidant enzymes (peroxidase and superoxide dismutase), glutathione peroxidase, glucose dehydrogenase, angiotensin-converting enzyme, cadherin, and calreticulin, were highly expressed in H. persikonus on the summer host, while a SCP GAPR-1-like family protein and a salivary sheath protein were highly expressed in the aphids on winter hosts. These results shed light on phenotypic plasticity in host utilization and seasonal adaptation of aphids.