ASSOCIATION OF A 5S GENE-TRANSCRIPTION FACTOR WITH 5S RNA AND ALTERED LEVELS OF THE FACTOR DURING CELL-DIFFERENTIATION
ASSOCIATION OF A 5S GENE-TRANSCRIPTION FACTOR WITH 5S RNA AND ALTERED LEVELS OF THE FACTOR DURING CELL-DIFFERENTIATION
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DOI：
10.1016/0092-8674(80)90160-9
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
HONDA, BM;ROEDER, RG
从非洲爪蟾卵巢中纯化了一种5S RNA基因体外转录所必需的蛋白因子TF IIIA。该因子（5S基因转录效应子）在免疫学、化学和功能标准上与未成熟卵母细胞中作为7S核糖核蛋白（RNP）复合物的5S RNA（基因产物）相关蛋白相同。卵母细胞成熟后，TF IIIA在未受精卵中不再能在功能上或免疫学上检测到，因为未受精卵在体外不合成5S RNA。TF IIIA不能在爪蟾体细胞提取物中被免疫学检测到，然而，爪蟾体细胞有效地转录5S基因。
A protein factor (TF IIIA) which is necessary for the accurate in vitro transcription of 5S RNA genes was purified from Xenopus ovaries. This factor (a 5S gene transcription effector) is identical by immunological, chemical and functional criteria to the protein associated with 5S RNA (the gene product) as a 7S ribonucleoprotein (RNP) complex in immature oocytes. After oocyte maturation, TF IIIA is no longer detectable functionally or immunologically in unfertilized eggs, which do not synthesize 5S RNA in vitro. TF IIIA cannot be immunologically detected in extracts from Xenopus somatic cells which, nevertheless, efficiently transcribe 5S genes.