Portosystemic shunt prevents apoptosis in rat intestinal mucosa caused by total hepatic ischemia
Portosystemic shunt prevents apoptosis in rat intestinal mucosa caused by total hepatic ischemia
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DOI：
10.1159/000079914
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发表时间：
2004-01-01
影响因子：
1.6
通讯作者：
Muhtaroglu, S
中科院分区：
文献类型：
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作者：
Bedirli, A;Sakrak, O;Muhtaroglu, S
背景：由于全肝缺血（THI）引起的长时间内脏充血已被证明可诱导肠粘膜损伤。本研究旨在探讨门体分流术（PSS）对THI大鼠肠黏膜细胞凋亡的保护作用。研究方法：将成年雄性Wistar大鼠随机分为3组：对照组、THI组、PSS组。再灌注1、2和6 h后处死大鼠。检测各组大鼠血清肝酶、肠组织形态学、肠组织丙二醛（MDA）含量及DNA片段化。此外，还测量了7天存活率。结果：THI组的7天生存率为50%，PSS组的7天生存率为90%（P < 0.01）。THI组和PSS组血清AST和ALT水平在再灌注后迅速升高，2h达高峰。THI组再灌注1h和2h后MDA水平显著高于对照组（P < 0.001）。THI组再灌注后1小时DNA片段百分比的增加达到峰值。PSS可减少肠粘膜DNA的断裂，并保持肠粘膜的宏观和微观外观。结论：门脉阻断引起的内脏淤血可增加大鼠肠黏膜细胞凋亡。PSS在抵消全肝缺血的主要负面影响方面非常有效。版权所有（C）2004 S. Karger AG，巴塞尔。
Background: Prolonged splanchnic congestion due to total hepatic ischemia (THI) has been shown to induce damage to the intestinal mucosa. The present study was conducted to examine whether the protective effect of portosystemic shunt (PSS) can be seen on apoptosis of intestinal mucosa in a rat model of THI. Methods: Adult male Wistar rats were divided into the following 3 groups: control group; the THI group underwent THI for 30 min, and the PSS group was subjected to THI for 30 min with PSS. Rats were killed after 1, 2, and 6 h of reperfusion. For each time point, levels of serum liver enzymes, intestinal morphology, malondialdehyde (MDA) contents and DNA fragmentation in intestinal tissue were determined. In addition, the 7-day survival rate was measured. Results: The 7-day survival rate of THI group remained at 50%, whereas that of PSS group was significantly higher at 90% (p < 0.01). Serum AST and ALT levels of the THI and PSS groups rapidly increased after reperfusion, reaching peak values at 2 h. MDA levels after 1 and 2 h of reperfusion in the THI group were significantly increased as compared with the control group p < 0.001). Increases in the percentage of fragmented DNA peaked 1 h after reperfusion in the THI group. PSS resulted in the reduction of DNA fragmentation and preserved the macroscopic and microscopic appearance of the intestinal mucosa. Conclusions: Splanchnic congestion due to portal occlusion increased apoptosis in the rat intestinal mucosa. PSS is very effective in counteracting the principal negative effects of total hepatic ischemia. Copyright (C) 2004 S. Karger AG, Basel.