Melatonin concentrations in the two jugular veins, and relationship with the seasonal reproductive activity in goats
Melatonin concentrations in the two jugular veins, and relationship with the seasonal reproductive activity in goats
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DOI：
10.1016/j.theriogenology.2010.02.005
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发表时间：
2010-07-15
期刊：
影响因子：
2.8
通讯作者：
Malpaux, B.
中科院分区：
文献类型：
--
作者：
Zarazaga, L. A.;Celi, I.;Malpaux, B.
作者研究了褪黑激素浓度是否在两个颈静脉之间变化，以及绝对（夜间）或相对（夜间/昼夜比率）血浆褪黑激素浓度是否与Payoya山羊的发情或排卵活动测量的季节性生殖活动相关。每天使用系围裙的雄性动物测试发情活性-每周两次对血浆取样以测定孕酮。在每年的春分和夏至，在通过静脉穿刺同时采集的颈静脉样品中研究褪黑激素血浆浓度。分别在每只山羊的3个和2个血浆样品中评估来自每个颈静脉的夜间和白天血浆褪黑激素浓度，在每个时间段以每小时的间隔进行测量。没有观察到两个静脉之间的褪黑激素浓度的差异。而颈静脉与动物之间的褪黑素浓度存在显著的交互作用（P <0.001）。取样周期对褪黑激素浓度无影响，取样周期之间的相关系数很高。分析表明，褪黑激素的绝对浓度和相对浓度与排卵/发情活动开始或结束的日期无关。我们得出结论：在山羊中（1）褪黑激素浓度在同一个体的颈静脉之间高度可变，但在一般群体中不是，（2）褪黑激素浓度对于每个个体是高度可重复的，并且（3）褪黑激素浓度的绝对和相对幅度与地中海山羊的季节性繁殖活动无关（C）2010 Elsevier Inc.版权所有
The authors investigated whether melatonin concentrations vary between the two jugular veins and whether absolute (nocturnal) or relative (nocturnal/diurnal ratio) plasma melatonin concentrations are associated with seasonal reproductive activity measured by oestrus or ovulatory activity in Payoya goats Thirty-two adult Payoya goats were penned under natural photoperiod. Oestrus activity was tested daily using aproned males-twice a week plasma was sampled for progesterone Melatonin plasma concentrations were studied at each equinox and solstice of the year in Jugular samples taken simultaneously by venipuncture Nocturnal and diurnal plasma melatonin concentrations from each jugular vein were assessed in 3 and 2 plasma samples per goat, respectively, taken at hourly intervals in each period No differences in melatonin concentrations between the two veins were observed. but there was a significant interaction (P < 0 001) between jugular vein and animal in nocturnal melatonin concentrations. There was no effect of sampling period on melatonin concentrations and the coefficient of correlation between sampling periods was very high The analyses performed indicated that neither absolute nor relative melatonin concentrations were related with the dates of onset or end of ovulatory/oestrus activity Therefore, we concluded that in goats (1) melatonin concentrations are highly variable between jugular veins in the same individual but not in the general population, (2) melatonin concentrations are highly repeatable for each individual, and (3) absolute and relative amplitudes of melatonin concentrations are not linked to the seasonal breeding activity in Mediterranean goats (C) 2010 Elsevier Inc. All rights reserved