Increased expression of the histamine H4 receptor following differentiation and mediation of the H4 receptor on interleukin-8 mRNA expressio n in HaCaT keratinocytes
Increased expression of the histamine H4 receptor following differentiation and mediation of the H4 receptor on interleukin-8 mRNA expressio n in HaCaT keratinocytes
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HaCaT 角质形成细胞中 H4 受体分化和介导白细胞介素 8 mRNA 表达后，组胺 H4 受体表达增加
DOI：
10.1111/exd.12313
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发表时间：
2014
影响因子：
3.6
通讯作者：
Koichi Ueno
中科院分区：
文献类型：
--
作者：
Eriko Suwa;Katsunori Yamaura;Shiori Sato;Koichi Ueno
最近的体内研究表明，组胺H4受体参与瘙痒和皮肤炎症。我们以前报道过，H4受体拮抗剂减弱了特应性皮炎实验模型中的抓挠行为并改善了皮肤病变。我们还报道了H4受体在人表皮组织中的表达。在这项研究中，我们研究了H4受体mRNA的表达和功能的受体在培养系统中，模仿体内炎症的HaCaT人角质形成细胞系。在分化后的HaCaT细胞中观察到H4受体的表达增加。用组胺和TNFα处理HaCaT细胞可增强白细胞介素（IL）-8的mRNA表达。H4受体拮抗剂JNJ 7777120可显著抑制这些表达增加。我们的研究结果表明，组胺和TNFα可能通过刺激角质形成细胞中的H4受体来增强IL-8 mRNA的表达。
Recentin vivostudies have demonstrated involvement of the histamine H4 receptor in pruritus and skin inflammation. We previously reported that an H4 receptor antagonist attenuated scratching behaviour and improved skin lesions in an experimental model of atopic dermatitis. We also reported the expression of the H4 receptor in human epidermal tissues. In this study, we investigated the expression of H4 receptor mRNA and the function of the receptor in a culture system that mimicsin vivoinflammation on the HaCaT human keratinocyte cell line. Increased expression of the H4 receptor was observed in HaCaT cells following differentiation. Treatment of HaCaT cells with histamine and TNFαenhanced the mRNA expression of interleukin (IL)‐8. These increases in expression were significantly inhibited by the H4 receptor antagonist JNJ7777120. Our results indicate that IL‐8 mRNA expression might be enhanced by histamine and TNFαvia H4 receptor stimulation in keratinocytes.