Assessment of a simplified method for counting leukocytic malaria pigment
Assessment of a simplified method for counting leukocytic malaria pigment
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DOI：
10.4269/ajtmh.2005.73.588
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发表时间：
2005-09-01
影响因子：
3.3
通讯作者：
Kremsner, PG
中科院分区：
文献类型：
--
作者：
Lell, B;Missinou, MA;Kremsner, PG
严重和致命的疟疾与外周吞噬细胞中疟原虫色素的增加有关。对这种关系的大规模研究受到这样一个事实的阻碍，即在厚的血涂片上识别和计数吞噬细胞是耗时的。区分哪些单核细胞是单核细胞，哪些粒细胞是中性粒细胞需要时间和仔细的训练。在这项研究中，我们评估了一种简化的方法，其中只计算含疟原虫色素的单核细胞和粒细胞的比例。对471名加蓬疟疾患儿的厚血片进行了评价。我们发现含疟原虫色素的单核细胞与单核细胞（r = 0.85）和中性粒细胞与多形核细胞（r = 0.93）的比例之间的线性关系和强相关性。这两种方法有相似的预测值，估计受试者工作特征曲线。这种简化的方法可以用来估计外周血中的红细胞外色素的量，我们认为，它可能特别适合于非常大的研究。
Severe and fatal malaria is associated with the increased presence of malaria hemozoin in peripheral phagocytes. Large studies of this relationship are hampered by the fact that identifying and counting phagocytes on thick blood smears is time consuming. Distinguishing which mononuclear cells are monocytes and which granulocytes are neutrophils requires time and careful training. In this study, we evaluated a simplified method in which only the proportions of hemozoin-containing mononuclear cells and granulocytes are counted. Thick blood films from 471 Gabonese children with malaria were evaluated. We found a linear relationship and a strong correlation between the proportions of hemozoin-containing monocytes versus mononuclear cells (r = 0.85) and neutrophils versus polymorphonuclear cells (r = 0.93), respectively. The two methods had similar predictive values, as estimated by receiver operating characteristics curves. This simplified method can be used to estimate the amount of extra-erythrocytic pigment in peripheral blood, and we suggest that it may be particularly suitable for very large studies.