Osteocalcin expression in young and aged dental pulps as determined by RT-PCR.
Osteocalcin expression in young and aged dental pulps as determined by RT-PCR.
复制标题
通过 RT-PCR 测定年轻和老年牙髓中骨钙素的表达。
DOI：
10.1016/s0099-2399(97)80186-0
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
MacDougall,M
中科院分区：
文献类型：
--
作者：
Ranly,DM;Thomas,HF;Chen,J;MacDougall,M
从年轻人（17-25岁）的第三磨牙或从>50岁的个体的磨牙获得牙髓，并通过RT-PCR检测骨钙素（OC）mRNA的表达。OC被选为牙髓活力的决定因素，因为它长期以来与牙齿和骨骼中硬组织基质的产生有关。为了比较的目的，通过建立OC/GAPDH比率，将每种果肉中OC的表达相对于其管家基因产物GAPDH标准化。这项研究表明，OC的表达，大概由成牙本质细胞谱系的细胞，不减少相对于现存的细胞群体。我们的研究结果表明，尽管体积和细胞数量减少，但老化牙齿的牙髓保留了牙本质沉积的能力，并具有抗龋齿和抗创伤的潜力。
Dental pulps were obtained from third molars of young adults (17–25 yr) or from molar teeth of individuals >50 yr of age and examined for the expression of osteocalcin (OC) mRNA by RT-PCR. OC was selected as a determinant of pulp vitality, because it has long been associated with the production of hard tissue matrix in teeth and bone. For comparative purposes, the expression of OC in each pulp was normalized relative to its housekeeping gene-product GAPDH by the establishment of a OC/GAPDH ratio. This study demonstrated that OC expression, presumably by cells of odontoblast lineage, does not diminish relative to the extant cell population. Our findings suggest, despite a reduction in volume and cell numbers, that the pulps of aging teeth retain a capacity for dentin deposition and a potential for caries and trauma resistance.