Food detection and preferences of the nudibranch mollusc Hermissenda crassicornis: experiments in a Y-maze.
Food detection and preferences of the nudibranch mollusc Hermissenda crassicornis: experiments in a Y-maze.
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裸鳃类软体动物 Hermissenda crassicornis 的食物检测和偏好：Y 型迷宫中的实验。
DOI：
10.1086/bblv187n2p274
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kuzirian,AM
中科院分区：
文献类型：
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作者：
Tyndale,E;Avila,C;Kuzirian,AM
Hermissenda crassicornis（Escholtz，183 I）是一种裸鳃类软体动物，正在我们实验室饲养，作为学习和记忆研究的生物医学研究模型。本研究的目的是确定Hermissenda，一种多面手食肉动物（1），是否能够检测其猎物的化学活性，以及它是否表现出对某些食物的偏好。先前对其他裸鳃类动物的研究表明，它们有能力从远处发现猎物（2，3）。实验在35 cm宽X 70 cm长X 12 cm深的Y型迷宫中进行，环境海水的总平衡流速为1500-2500 ml/min（水深：每个臂3cm：17 cm宽）。一个均匀的，单向流动，实现了与上游准直器制成的苏打秸秆。染料测试证实了电流（即刺激）在迷宫两臂的整个宽度上是均匀的。水螅体的大黄鱼、被囊的玻璃海鞘和贻贝是测试的食物。这些物种在过去都曾在实验室中成功地用于喂养Hermissenda（1，4）; Ciona被去除了被膜，贻贝没有壳。将食物放置在Y形迷宫的一侧，另一侧保持空的。对于Y-迷宫实验，管形藻是整体呈现的;玻璃海鞘以三种方式呈现：整体、仅内脏和内脏切成碎片;贻贝是整体但没有壳。在测试管藻的茎和水螅之间的偏好的试验中，水螅体的碎片包含在500 μ π ι网眼袋中，Y-迷宫的每一侧一个。
Hermissenda crassicornis (Eschscholtz, 183 I), a nudibranch mollusc, is being reared in our laboratory as a biomedical research model for learning and memory studies. The aim of the present study was to determine whether Hermissenda, a generalist carnivore (l), is able to detect its prey chemotactically, and whether it displays a preference for certain foods. Previous studies on other nudibranchs demonstrated their ability to detect prey from a remote distance (2, 3). Experiments were carried out in a 35 cm wide X 70 cm long X 12 cm deep Y-maze with a total equilibrated flow of 1500-2500 ml/min of ambient seawater (water depth: 3 cm each arm: 17 cm wide). A uniform, unidirectional flow was achieved with an upstream collimator made of soda straws. Dye tests confirmed the uniformity of the currents (ie, stimulus) across the full width of both arms of the maze.The hydroid Tubularia crocea, the tunicate Ciona intestinalis, and the mussel Mytilus edulis were the foods tested. These species have all been used successfully in the past to feed Hermissenda in the laboratory (1, 4); Ciona was presented with the tunic removed, and mussels were presented without shells. Food was placed on one side of the Y-maze, with the other side remaining empty. For Y-maze experiments, Tubularia was presented whole; Ciona in three ways: whole, viscera only, and viscera cut into pieces; mussels were whole but without shells. In trials testing for a preference between stalks and polyps of Tubularia, the pieces of the hydroid were contained in 500~ pm mesh bags, one on each side of the Y-maze.