ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
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DOI：
10.1083/jcb.127.1.129
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发表时间：
1994-10
期刊：
影响因子：
--
通讯作者：
Meyer DI
中科院分区：
文献类型：
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作者：
Clark SW;Meyer DI
作为我们正在进行的努力，以了解脊椎动物centractin的功能作用的一部分，我们已经确定了一个新成员的肌动蛋白相关的蛋白质家族的酵母酿酒酵母使用PCR为基础的方法。与目前酵母中肌动蛋白相关蛋白的命名一致，我们建议将此位点命名为ACT 3。Act 3 p的一级氨基酸序列与犬和人α-centractin最相似（73%相似性/54%同一性）。基因组克隆的序列表明ACT 3与染色体VIII上的FUR 1相邻并相对于其收敛地转录。分子遗传学分析表明，ACT 3是由一个单一的基因，从相应的mRNA表达在一个较低的水平相比，ACT 1。四分体分析杂合子窝藏ACT 3编码区的TRP 1取代表明ACT 3是在正常条件下，在极端的温度和渗透压的生长是不必要的。然而，在14摄氏度的生长表明纺锤体方向缺陷类似于最近描述的酵母在肌动蛋白，微管蛋白或细胞质动力蛋白中携带突变的表型。综上所述，我们的数据表明ACT 3是S。脊椎动物centractin的酿酒酵母同源物。
As part of our ongoing efforts to understand the functional role of vertebrate centractins, we have identified a new member of the actin- related family of proteins in the yeast Saccharomyces cerevisiae using a PCR-based approach. Consistent with the current nomenclature for actin-related proteins in yeast, we propose to denote this locus ACT3. The primary amino acid sequence of Act3p is most similar to canine and human alpha-centractin (73% similarity/54% identity). The sequence of a genomic clone indicates ACT3 lies adjacent to and is transcribed convergently with respect to FUR1 on chromosome VIII. Molecular genetic analysis indicates ACT3 is represented by a single gene from which the corresponding mRNA is expressed at a low level compared to ACT1. Tetrad analysis of heterozygotes harboring a TRP1 replacement of the ACT3- coding region indicates ACT3 is nonessential for growth under normal conditions and at extremes of temperature and osmolarity. However, growth at 14 degrees C indicates a spindle orientation defect similar to phenotypes recently described for yeast harboring mutations in actin, tubulin, or cytoplasmic dynein. Taken together, our data suggest that ACT3 is the S. cerevisiae homologue of vertebrate centractins.