Tuning the selectivity of a commercial cyanine nucleic acid dye for preferential sensing of hybrid telomeric G-quadruplex DNA
Tuning the selectivity of a commercial cyanine nucleic acid dye for preferential sensing of hybrid telomeric G-quadruplex DNA
复制标题
调整商业花青核酸染料的选择性以优先检测混合端粒 G-四链体 DNA
DOI：
10.1016/j.snb.2018.03.125
复制
发表时间：
2018-08-01
影响因子：
8.4
通讯作者：
Tan, Jia-Heng
中科院分区：
文献类型：
--
作者：
Wang, Yu-Qing;Hu, Ming-Hao;Tan, Jia-Heng
近年来，G-四链体在诊断和治疗方面的应用越来越受到人们的关注。这就要求开发高选择性的探针，可以区分大量的G-四链体中的特定结构。然而，到今天为止，很少有探针显示出令人满意的选择性的一个特定的G-四链体结构。在这项研究中，我们设计了一个新的探针称为TO-BTZ通过引入一个额外的苯并噻唑部分的商业菁染料噻唑橙子（TO）。TO的这种修饰通过特异性靶向在5 '端G-四链体附近形成的独特结合位点，显著地调节了其对杂合端粒G-四链体DNA的选择性。这种特异性相互作用将TO-BTZ H-聚集体分解为其单体，并进一步阻碍TO-BTZ单体的分子旋转，从而导致荧光增强。此外，我们观察到TO-BTZ具有在细胞中感知端粒G-四链体的潜力。总之，这项工作将揭示寻找新一代的探针，可以区分不同的G-四链体结构。(C)2018 Elsevier B. V.版权所有。
Recent years have witnessed an ever-increasing interest in G-quadruplexes for diagnostic and therapeutic applications. This calls for the development of highly selective probes that can differentiate a particular structure among large quantities of G-quadruplexes. However, to this day, there are few probes that show satisfactory selectivity for a particular G-quadruplex structure. In this study, we engineered a new probe called TO-BTZ by introducing an additional benzothiazole moiety on a commercial cyanine dye thiazole orange (TO). Such a modification of TO significantly tuned its selectivity toward the hybrid telomeric G-quadruplex DNA by specifically targeting the unique binding site formed near the 5'-end G-quartet. Such specific interactions disassembled the TO-BTZ H-aggregate to its monomers and further hindered the molecular rotation of the TO-BTZ monomer, thus leading to fluorescence enhancement. Furthermore, we observed that TO-BTZ has the potential to sense telomeric G-quadruplexes in cells. Taken together, this work will shed light on the search for a new generation of probes that can distinguish between different G-quadruplex structures. (C) 2018 Elsevier B.V. All rights reserved.