Characterization of gangliosides from bovine erythrocyte membranes.
Characterization of gangliosides from bovine erythrocyte membranes.
复制标题
牛红细胞膜神经节苷脂的表征。
DOI：
--
复制
发表时间：
1978
影响因子：
4.8
通讯作者：
Y. Li
中科院分区：
文献类型：
--
作者：
J. Chien;S. Li;R. Laine;Y. Li
从牛红细胞膜中分离出两种含葡萄糖胺的神经节苷脂，唾液酸基六糖基神经酰胺。两种神经节苷脂均被从产气荚膜梭菌中分离出的神经氨酸酶水解，成为中性六糖基神经酰胺。基于甲基化糖的连续酶水解和气相色谱-质谱分析结果，这两种神经节苷脂的结构显示为 NeuAcalpha2 导致 3Galbeta1 导致 4GlcNAcbeta1 导致 3Galbeta1 导致 4GlcNAcbeta1 导致 3Galbeta1 导致 4Glc-神经酰胺，NeuGcalpha2 导致 3Galbeta1 导致4GlcNAcbeta1 分别导致 3Galbeta1 导致 4GlcNAcbeta1 导致 3Galbeta1 导致 4Glc-神经酰胺。此外，在牛红细胞中还发现了N-乙酰基-和N-羟氨酰基乳糖-N-新四糖神经酰胺，以及N-乙酰基-和N-羟氨酰基乳糖基神经酰胺。这两种神经节苷脂中的主要脂肪酸是C 22:0 和C 24:0。 C-18 鞘氨醇是检测到的主要碱基。
Two glucosamine-containing gangliosides, sialosylhexaglycosylceramides, were isolated from bovine erythrocyte membranes. Both gangliosides were hydrolyzed by neuraminidase isolated from Clostridium perfringens to become neutral hexaglycosylceramides. Based on the results of sequential enzymatic hydrolysis and gas chromatography-mass spectrometric analyses of the methylated sugars, the structures of these two gangliosides were shown to be NeuAcalpha2 leads to 3Galbeta1 leads to 4GlcNAcbeta1 leads to 3Galbeta1 leads to 4GlcNAcbeta1 leads to 3Galbeta1 leads to 4Glc-ceramide and NeuGcalpha2 leads to 3Galbeta1 leads to 4GlcNAcbeta1 leads to 3Galbeta1 leads to 4GlcNAcbeta1 leads to 3Galbeta1 leads to 4Glc-ceramide, respectively. In addition, N-acetyl- and N-glycolylneuraminosyllacto-N-neotetraosylceramides, and N-acetyl- and N-glycolylneuraminosyllactosylceramides were also found in bovine erythrocytes. The predominant fatty acids in these two gangliosides were C 22:0 and C 24:0. C-18 sphingosine was the major base detected.