Oral administration of heat-killed Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice
Oral administration of heat-killed Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice
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DOI：
10.1016/j.intimp.2009.04.015
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发表时间：
2009-08-01
影响因子：
5.6
通讯作者：
Yoshikai, Yasunobu
中科院分区：
文献类型：
--
作者：
Maeda, Naoyoshi;Nakamura, Risa;Yoshikai, Yasunobu
我们先前报道了热灭活的植物乳杆菌L-137(HK-LP)在体外和体内刺激巨噬细胞/树突状细胞产生辅助性T细胞(Th)1相关的细胞因子。在此，我们研究了口服HK-LP对小鼠流感病毒感染的保护作用。C57BL/6小鼠于第7天至第7天经口给予HK-LP，第0天以100pfu经鼻感染流感病毒A/FM/1/47(H1N1，小鼠适应株)。与PBS对照组相比，HK-LP组小鼠存活时间明显延长。在流感病毒感染后早期，HK-LP治疗组小鼠肺内病毒滴度明显低于对照组。经HK-LP处理的小鼠在感染流感后的血清中检测到较高水平的干扰素-β，而对照组则未检测到干扰素-β。我们的结果表明，HK-LP是一种有效的干扰素-β诱导剂，对预防流感感染是有用的。(C)2009爱思唯尔B.V.保留所有权利。
We have previously reported that heat-killed Lactobacillus plantorum L-137 (HK-LP) stimulates macrophage/dendritic cells to produce T helper (Th) 1-related cytokines in vitro and in vivo in mice. We here examined the effect of oral administration of HK-LP on protection against influenza virus infection in mice. C57BL/6 mice were orally given HK-LP from day -7 to 7 and intranasally infected with influenza virus A/FM/1/47 (H1N1, a mouse-adapted strain) at 100 pfu on day 0. The survival time was significantly prolonged in mice treated with HK-LP than that in mice treated with PBS as controls. The viral titers in the lung were significantly lower in mice treated with HK-LP than controls at the early stage after influenza virus infection. An appreciable level of interferon (IFN)-beta was detected in the serum of mice treated with HK-LP, while no IFN-beta was detected in controls after influenza infection. Our results suggest that HK-LP, a potent IFN-beta inducer, is useful for prevention against influenza infection. (C) 2009 Elsevier B.V. All rights reserved.