Effects of Tongxinluo on myocardial fibrosis in diabetic rats
Effects of Tongxinluo on myocardial fibrosis in diabetic rats
复制标题
DOI：
10.1016/j.jcma.2015.06.022
复制
发表时间：
2016-03-01
影响因子：
3
通讯作者：
Zhang, Zhimian
中科院分区：
文献类型：
--
作者：
Wang, Xiaomei;Mu, Changzheng;Zhang, Zhimian
背景：探讨通心络对糖尿病大鼠心肌纤维化的影响及其可能的作用机制。方法：建立糖尿病大鼠模型，将其分为对照组、糖尿病组和通心络组。观察心功能和心肌间质胶原体积分数，测定转化生长因子β 1 （tgf - β(1)）、Smad(3)、Smad(7)蛋白和mRNA表达水平。结果：与对照组相比，糖尿病组心功能明显异常，胶原体积分数、tgf - β(1)、Smad(3) mRNA表达水平升高。而Smad7蛋白和mRNA的表达水平较低。通心络组上述指标均有改善。结论：通心络对糖尿病大鼠心肌纤维化有一定的防治作用。可能通过介导大鼠心肌细胞中tgf - β(1)、Smad(3)、Smad(7)的表达，减少糖尿病大鼠心肌纤维化的发生。中华医学会著作权所有2015爱思唯尔台湾有限责任公司出版。
Background: The aim of this study was to explore the effect of Tongxinluo on myocardial fibrosis in diabetic rats and its possible mechanism of action.Methods: Diabetic rat models were established and then divided into three groups: control, diabetes, and Tongxinluo groups. Heart function and myocardial interstitial collagen volume fraction were investigated, and the protein and mRNA expression levels of transforming growth factor beta 1 (TGF-beta(1)), Smad(3), and Smad(7) were measured.Results: Heart function was clearly abnormal in the diabetes group compared with that in the control group, and the collagen volume fraction and mRNA expression levels of TGF-beta(1) and Smad(3) were higher. However, the protein and mRNA expression levels of Smad7 were lower. In the Tongxinluo group, it was observed that these indicators were improved.Conclusion: Tongxinluo was effective for the prevention and treatment of myocardial fibrosis in diabetic rats. It probably mediates the expressions of TGF-beta(1), Smad(3), and Smad(7) in rat cardiomyocytes to reduce the occurrence of myocardial fibrosis in diabetic rats. Copyright (C) 2015, the Chinese Medical Association. Published by Elsevier Taiwan LLC.