Conditional Effect of the Deletion of eshA on Streptomycin Production in Streptomyces griseus IFO13350
Conditional Effect of the Deletion of eshA on Streptomycin Production in Streptomyces griseus IFO13350
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DOI：
10.3209/saj.saj240205
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发表时间：
2010-12
期刊：
影响因子：
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通讯作者：
Takeaki Tezuka;Y. Ohnishi;S. Horinouchi
中科院分区：
文献类型：
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作者：
Takeaki Tezuka;Y. Ohnishi;S. Horinouchi
eshA 基因最初被发现编码灰色链霉菌 NRRL B-2682 的沉水孢子形成过程中产孢菌丝延伸所需的蛋白质。据报道，灰色链霉菌 IFO13189 的 eshA 破坏菌株在链霉素生产和气生菌丝体形成方面存在条件性缺陷。我们之前的转录组分析表明，AdpA（形态和生理分化的全局转录调节因子）在 S. griseus IFO13350 中诱导 eshA (SGR1270) 转录。在这里，我们检查了 AdpA 对 eshA 的转录调控以及 eshA 在 S. griseus IFO13350 的形态和生理分化中的参与。通过 S1 核酸酶图谱进行的转录分析表明 eshA 在固体培养基上的整个生长过程中进行转录。相反，在 adpA 缺失突变体中没有检测到 eshA 转录。体外，带有 His 标签的重组 AdpA 与 eshA 启动子上游区域结合。然而，AdpA结合序列的突变并不影响eshA在体内的转录，表明AdpA间接激活eshA转录。在 TSB 平板上生长的 eshA 缺失突变体的链霉素产量低于野生型菌株。然而，eshA缺失突变体在各种培养基上的生长和形成气生菌丝体和孢子的时间过程与野生型菌株相同。
The eshA gene was originally found to encode a protein required for the extension of sporogenic hyphae during submerged spore formation in Streptomyces griseus NRRL B-2682. An eshA-disrupted strain of S. griseus IFO13189 was reported to be conditionally deficient in streptomycin production and aerial mycelium formation. Our previous transcriptomic analyses indicated that AdpA, a global transcriptional regulator of morphological and physiological differentiation, induced eshA (SGR1270) transcription in S. griseus IFO13350. Here, we examined the transcriptional regulation of eshA by AdpA and the involvement of eshA in the morphological and physiological differentiation of S. griseus IFO13350. Transcriptional analysis by S1 nuclease mapping showed that eshA was transcribed throughout growth on solid medium. In contrast, no eshA transcription was detected in an adpA deletion mutant. Recombinant His-tagged AdpA bound to a region upstream from the eshA promoter in vitro. However, mutation of the AdpA-binding sequence did not affect the transcription of eshA in vivo, indicating that AdpA indirectly activates eshA transcription. Streptomycin production by an eshA deletion mutant grown on TSB plates was lower than that of the wild-type strain. However, the eshA deletion mutant grew and formed aerial mycelia and spores following the same time course as the wild-type strain on various media.