DETERMINATION OF D-ASPARTATE AND L-ASPARTATE IN AMINO-ACID MIXTURES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH A CHIRAL ADDUCT OF O-PHTHALDIALDEHYDE
DETERMINATION OF D-ASPARTATE AND L-ASPARTATE IN AMINO-ACID MIXTURES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH A CHIRAL ADDUCT OF O-PHTHALDIALDEHYDE
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DOI：
10.1016/0003-2697(84)90106-4
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
ASWAD, DW
中科院分区：
文献类型：
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作者：
ASWAD, DW
本文提出了一种同时测定混合氨基酸中D-和L-天冬氨酸的方法。该方法涉及衍生的混合物与手性荧光，然后通过高效液相色谱反相柱。所用的荧光团是邻苯二甲醛与光学活性硫醇N-乙酰基-L-半胱氨酸的加合物。该方法的灵敏度和准确度与邻苯二甲醛与非手性硫醇2-巯基乙醇加合物的方法相当。在存在100倍过量的L-天冬氨酸的情况下，可以准确地检测到5皮摩尔的D-天冬氨酸，总分析时间（包括衍生化）为10分钟。
A sensitive and convenient method for the simultaneous determination of D- and L-aspartic acid in amino acid mixtures is described. The method involves derivatization of the mixture with a chiral fluorogen, followed by high-performance liquid chromatography on a reverse-phase column. The fluorogen used is an adduct of o-phthaldialdehyde with an optically active thiol, N-acetyl-L-cysteine. The sensitivity and accuracy of this method is similar to that using adducts of o-phthaldeialdehyde with the achiral thiol, 2-mercaptoethanol. Five picomoles of D-aspartate can be accurately detected in the presence of a 100-fold excess of L-aspartate with a total analysis time (including derivatization) of 10 min.