Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe
Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe
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双光子荧光探针揭示小鼠中风模型中硫氧还蛋白还原酶功能丧失
DOI：
10.1039/d0cc05900e
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发表时间：
2020
影响因子：
4.9
通讯作者：
Fang Jianguo
中科院分区：
文献类型：
--
作者：
Qu Yuan;Zhao Jintao;Gao Hao;Zhong Miao;Li Xinming;Zhang Fang;Chen Yaxiong;Gan Lu;Hu Guodong;Zhang Hong;Zhang Shengxiang;Fang Jianguo
硫氧还蛋白还原酶（TrxR）在调节细胞中的氧化还原稳态中是关键的。我们报道了第一个哺乳动物TrxR的双光子荧光探针（TP-TRFS）。TP-TRFS在识别TrxR方面保持了高特异性。更重要的是，TP-TRFS的双光子吸收特性使其能够在体内使用。在TP-TRFS的帮助下，首次在中风小鼠模型的脑中观察到TrxR功能的显著下降，提供了TrxR功能障碍与中风的机制联系。
Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.