Impact of high-intensity interval training on tendon related gene expression in rat Achilles tendon
Impact of high-intensity interval training on tendon related gene expression in rat Achilles tendon
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高强度间歇训练对大鼠跟腱肌腱相关基因表达的影响
DOI：
10.1016/j.bbrc.2023.03.076
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发表时间：
2023
影响因子：
3.1
通讯作者：
Tetsuya Izawa
中科院分区：
文献类型：
--
作者：
Yoshifumi Tsuchiya;Hisashi Takakura;Seita Osawa;Tetsuya Izawa
与有限的体力活动相关的制动或衰老可导致肌腱的功能退化，这已成为一个重要的公共卫生问题。因此，运动训练对保存肌腱功能的作用受到越来越多的研究。运动训练使肌肉和肌腱承受反复的机械应力，在体外研究中，重复的机械负荷刺激肌腱细胞对细胞外基质和肌腱功能特性的变化做出反应。然而，尽管几种类型的运动训练已经被证明在保护肌腱功能方面是有效的，但还没有研究调查高强度间歇训练(HIIT)的影响，高强度间歇训练涉及组成具有高功率输出的短回合运动。在这里，我们通过测量大鼠跟腱中的mRNA表达来确定HIIT计划是否促进了张力形成的进展。将16只大鼠随机分为安静对照组(CON，n=8)和HIIT组(n=8)。HIIT组大鼠在跑步机上进行训练，训练量递增(跑速度、跑组数、跑斜度)，每周训练5天，共9周。HIIT组大鼠体重和不同类型脂肪重量明显减少，不同类型肌肉重量明显增加。实时定量逆转录聚合酶链式反应分析显示，与CON组相比，HIIT组肌腱相关基因Tnxb、OPn和Tgfb1的mRNA表达上调。HIIT组胶原相关Dcnn和Fmodin基因表达的交联度明显高于CON组。这些结果表明，HIIT可诱导大鼠跟腱的肌腱发生进程，并刺激胶原纤维之间的交联形成。
Immobilization or aging associated with limited physical activity can lead to the functional deterioration of tendons, which has become an important public health concern. Therefore, growing research is focused on the effect of exercise training on preserving tendon function. Exercise training subjects muscles and tendons to repeated mechanical stress, andin vitrostudies have revealed that repetitive mechanical loading stimulates tendon cell responses to changes in the extracellular matrix and functional properties of the tendon. However, although several types of exercise training have been shown to be effective in preserving tendon function, no studies have investigated the impact of high-intensity interval training (HIIT), which involves composing short bouts of exercise with high-power output. Here, we determined whether the HIIT program enhanced tenogenic progressions by measuring the mRNA expression in rat Achilles tendons. Sixteen rats were randomly assigned into either a sedentary control group (Con, n = 8) or an HIIT group (n = 8). Rats in the HIIT group performed the program with treadmill running; the training volume was incremental (running speed, number of sets, and inclination), and training was conducted 5 days per week for 9 weeks. The rats in the HIIT group exhibited a marked decrease in the body weight and different types of fat weights, and a marked increase in different types of muscle weights. Real-time reverse transcription polymerase chain reaction analysis revealed that mRNA expressions of tendon-related genesTnxb,Opn, andTgfb1were upregulated in the HIIT group compared to that in the Con group. Cross-links in mRNA expressions of collagen-relatedDcnandFmodin the HIIT group tended to be higher than in those Con group. These results suggest that HIIT induces initiation of tenogenic progression and stimulation of cross-link formation between collagen fibrils in rat Achilles tendons.