Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
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DOI：
10.1039/d0cs01551b
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发表时间：
2021-09-07
影响因子：
46.2
通讯作者：
Goss RJM
中科院分区：
文献类型：
--
作者：
Crowe C ;Molyneux S ;Sharma SV ;Zhang Y ;Gkotsi DS ;Connaris H ;Goss RJM
碳-卤键的酶促生成是自然界和合成化学家用来调节化合物的生物活性、生物利用度和反应性的强大策略，为选择性C-H官能化提供了机会。编码卤化酶的基因最近被证明超越了所有生命王国。这些酶将卤素原子安装到芳香族和活性较低的脂肪族底物中，实现使用合成方法通常具有挑战性的选择性。卤化酶发现和工程的重大进展提供了一个酶的工具箱，使这些催化剂能够在生物转化，合成生物学中随时使用，并与化学催化相结合，以实现后期C-H功能化。重点对底物范围，这篇综述概述了卤化酶的主要类别所采用的机制，同时在平行，它突出了利用酶卤化和化学催化C-H活化和多样化的结合的关键进展。概述了用于产生碳-卤键的酶工具及其通过酶和化学催化的组合在实现选择性C-H官能化中的用途。
The enzymatic generation of carbon–halogen bonds is a powerful strategy used by both nature and synthetic chemists to tune the bioactivity, bioavailability and reactivity of compounds, opening up the opportunity for selective C–H functionalisation. Genes encoding halogenase enzymes have recently been shown to transcend all kingdoms of life. These enzymes install halogen atoms into aromatic and less activated aliphatic substrates, achieving selectivities that are often challenging to accomplish using synthetic methodologies. Significant advances in both halogenase discovery and engineering have provided a toolbox of enzymes, enabling the ready use of these catalysts in biotransformations, synthetic biology, and in combination with chemical catalysis to enable late stage C–H functionalisation. With a focus on substrate scope, this review outlines the mechanisms employed by the major classes of halogenases, while in parallel, it highlights key advances in the utilisation of the combination of enzymatic halogenation and chemical catalysis for C–H activation and diversification. An overview of enzymatic tools for generation of carbon–halogen bonds and their use in enabling selective C–H functionalisation through combination of enzyme and chemo catalysis.
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影响因子：
18.3
作者：
Blasiak, Leah C.;Drennan, Catherine L.
通讯作者：
Drennan, Catherine L.
影响因子：
16.6
作者：
Chankhamjon, Pranatchareeya;Boettger-Schmidt, Daniela;Hertweck, Christian
通讯作者：
Hertweck, Christian
影响因子：
4
作者：
Bilyk, Oksana;Broetz, Elke;Luzhetskyy, Andriy
通讯作者：
Luzhetskyy, Andriy
影响因子：
2.9
作者：
Bitto, Eduard;Huang, Yu;Phillips, George N., Jr.
通讯作者：
Phillips, George N., Jr.
影响因子：
2.7
作者：
Baumann M;Baxendale IR
通讯作者：
Baxendale IR