3D Transcranial Ultrasound Localization Microscopy in the Rat Brain With a Multiplexed Matrix Probe
3D Transcranial Ultrasound Localization Microscopy in the Rat Brain With a Multiplexed Matrix Probe
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DOI：
10.1109/tbme.2021.3137265
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发表时间：
2022-07-01
影响因子：
4.6
通讯作者：
Couture, Olivier
中科院分区：
文献类型：
--
作者：
Chavignon, Arthur;Heiles, Baptiste;Couture, Olivier
目的：超声定位显微镜（乌尔姆）提供活体组织内微循环的深层图像。然而，它的实现在二维受到限制的高程投影和繁琐的逐面采集。容积乌尔姆解决了这些问题，并且可以在一次采集中以各向同性分辨率映射整个器官的脉管系统。然而，其最佳实施需要许多独立的采集通道，导致复杂的定制硬件。研究方法：在这篇文章中，我们实现了体积超声成像与多路复用32 × 32探头驱动的一个单一的商业超声扫描仪。我们提出并比较了三种不同的子孔径复用组合的定位显微镜在硅片和在体外与微泡流在一个运河。最后，我们评估了大鼠脑微血管造影的方法。“轻”组合允许比“全”组合更高的最大体积速率，同时保持视场和分辨率。结果如下：在大鼠脑中，使用专用超声序列在7分钟内采集了100，000个体积，并显示直径低至31 μ m的血管，流速为4.3 mm/s至28.4 mm/s。结论：这项工作证明了在活体大鼠大脑中以前所未有的分辨率进行完整血管造影的能力，该方法通过完整的头骨进行简单而轻便的设置。意义：我们预见它可能有助于使3D乌尔姆在临床前和临床研究中普及。
Objective: Ultrasound Localization Microscopy (ULM) provides images of the microcirculation in-depth in living tissue. However, its implementation in two-dimension is limited by the elevation projection and tedious plane-by-plane acquisition. Volumetric ULM alleviates these issues and can map the vasculature of entire organs in one acquisition with isotropic resolution. However, its optimal implementation requires many independent acquisition channels, leading to complex custom hardware. Methods: In this article, we implemented volumetric ultrasound imaging with a multiplexed 32 x 32 probe driven by a single commercial ultrasound scanner. We propose and compare three different sub-aperture multiplexing combinations for localization microscopy in silico and in vitro with a flow of microbubbles in a canal. Finally, we evaluate the approach for micro-angiography of the rat brain. The "light" combination allows a higher maximal volume rate than the "full" combination while maintaining the field of view and resolution. Results: In the rat brain, 100,000 volumes were acquired within 7 min with a dedicated ultrasound sequence and revealed vessels down to 31 mu m in diameter with flows from 4.3 mm/s to 28.4 mm/s. Conclusion: This work demonstrates the ability to perform a complete angiography with unprecedented resolution in the living rat's brain with a simple and light setup through the intact skull. Significance: We foresee that it might contribute to democratize 3D ULM for both preclinical and clinical studies.