Association of decreased serum sTREM-1 level with the severity of coronary artery disease: Inhibitory effect of sTREM-1 on TNF-α- and oxLDL-induced inflammatory reactions in endothelial cells.
Association of decreased serum sTREM-1 level with the severity of coronary artery disease: Inhibitory effect of sTREM-1 on TNF-α- and oxLDL-induced inflammatory reactions in endothelial cells.
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血清 sTREM-1 水平降低与冠状动脉疾病严重程度的关联：sTREM-1 对 TNF- 和 oxLDL 诱导的内皮细胞炎症反应的抑制作用
DOI：
10.1097/md.0000000000004693
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发表时间：
2016-09
期刊：
影响因子：
1.6
通讯作者：
Tao R
中科院分区：
文献类型：
--
作者：
Dai D;Xiong W;Fan Q;Wang H;Chen Q;Shen W;Zhang R;Ding F;Lu L;Tao R
骨髓细胞表达的可溶性触发受体1（斯特雷姆-1）与自身免疫性疾病和炎症反应密切相关。我们的目的是研究血清斯特雷姆-1是否与冠状动脉疾病（CAD）有关，并在细胞实验中评估斯特雷姆-1的生物学效应。这项横断面研究包括263例连续的冠状动脉造影证实的CAD患者，这些患者入院接受CAD诊断和介入治疗（CAD组），162例无CAD的参与者作为对照（对照组）。测定所有受试者的血清斯特雷姆-1和高敏C反应蛋白（hsCRP）水平。在细胞实验中，在人脐静脉内皮细胞（HUVECs）中评估斯特雷姆-1对肿瘤坏死因子-α（TNF-α）或氧化低密度脂蛋白（oxLDL）诱导的炎症反应的影响。冠心病患者血清斯特雷姆-1水平显著低于对照组（P <0.001）。 斯特雷姆-1值与病变冠状动脉数量（斯皮尔曼r =-0.413，P <0.001）和Gensini评分表示的严重程度（Pearson r =-0.336，P <0.001）相关。 多因素Logistic回归分析显示，斯特雷姆-1降低是冠心病的独立危险因素（OR = 0.428，P <0.001）。 在细胞实验中，重组斯特雷姆-1蛋白浓度依赖性地抑制TNF-α或oxLDL诱导的HUVECs表达IL-1β、IL-6、TNF-α、VCAM-1和ICAM-1。这项研究表明，血清斯特雷姆-1水平降低与CAD的存在和严重程度显著相关。斯特雷姆-1抑制内皮细胞的炎症反应，提示其可能是一种潜在的血管保护因子。
Supplemental Digital Content is available in the text Soluble triggering receptor expressed on myeloid cells 1 (sTREM-1) is closely involved in autoimmune diseases and inflammatory reactions. We aimed to investigate whether serum sTREM-1 is related to coronary artery disease (CAD) and to evaluate the biological effects of sTREM-1 in cell experiments. This cross-sectional study included 263 consecutive patients with angiographically documented CAD, who were admitted for diagnosis and interventional treatment of CAD (CAD group), with 162 participants without CAD serving as controls (control group). Serum levels of sTREM-1 and high sensitivity C reactive protein (hsCRP) were determined in all participants. In cell experiments, the influence of sTREM-1 on tumor necrosis factor-α (TNF-α)- or oxidized low-density lipoprotein (oxLDL)-induced inflammatory reactions was evaluated in human umbilical vein endothelial cells (HUVECs). Serum level of sTREM-1 was significantly lower in CAD patients than in controls (P < 0.001). sTREM-1 values were related to the number of diseased coronary arteries (Spearman r = −0.413, P < 0.001) and the severity represented by Gensini score (Pearson r = −0.336, P < 0.001). Multivariable logistic regression analysis revealed that decreased sTREM-1 were independent determinants of CAD (OR = 0.428, P < 0.001). In cell experiments, recombinant sTREM-1 protein concentration-dependently inhibited the expression of IL-1β, IL-6, TNF-α, VCAM-1, and ICAM-1 induced by TNF-α or oxLDL in HUVECs. This study demonstrates that decreased serum sTREM-1 level is significantly associated with the presence and severity of CAD. sTREM-1 restrains inflammatory reaction in endothelial cells, suggesting that it might be a potential vascular protective factor.