Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity.
Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity.
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DOI：
10.1007/s10811-015-0619-0
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发表时间：
2016
影响因子：
3.3
通讯作者：
Pearson J
中科院分区：
文献类型：
--
作者：
Chater PI;Wilcox M;Cherry P;Herford A;Mustar S;Wheater H;Brownlee I;Seal C;Pearson J
使用比浊脂肪酶活性测定和上消化道的体外模拟评估了三种赫布里底褐海藻对脂肪酶活性的影响。试验了泡叶藻、墨角藻、水蓼的制剂；全海藻匀浆、碳酸钠提取物和乙醇提取物（沉淀和上清液分别进行测试）。所有提取物均显示出对脂肪酶的显着抑制作用，表明存在多种生物活性剂，可能包括藻酸盐、岩藻依聚糖和多酚。 F. vesiculosus 的完整匀浆提取物是最有效的脂肪酶抑制剂（IC50 = 0.119 mg mL-1），其次是乙醇上清液（IC50 = 0.159 mg mL-1），而乙醇沉淀和碳酸钠提取物的抑制作用相对较弱（分别为 IC50 = 0.360 mg mL-1 和 IC50 = 0.969 mg mL-1）。 对于 A. nodosum 和 P. canaliculata，乙醇颗粒的抑制作用最强（IC50 = 0.238 和 0.228 mg mL−1，分别）。这些抑制作用在模型肠道系统中得到了验证。本文提供的数据表明，使用海藻作为潜在的体重管理工具值得进一步研究。
The effect of three Hebridean brown seaweeds on lipase activity was assessed using a turbidimetric lipase activity assay and an in vitro simulation of the upper digestive tract. The preparations of Ascophyllum nodosum, Fucus vesiculosus, and Pelvetia canaliculata were tested; whole seaweed homogenate, sodium carbonate extract, and ethanol extracts (pellet and supernatant were tested separately). All extracts showed significant inhibition of lipase, suggesting multiple bioactive agents, potentially including alginates, fucoidans, and polyphenols. Whole homogenate extract of F. vesiculosus was the most potent inhibitor of Lipase (IC50 = 0.119 mg mL-1), followed by ethanol supernatant (IC50 = 0.159 mg mL-1) while ethanol pellet and sodium carbonate extract showed relatively weaker inhibition (IC50 = 0.360 mg mL-1 and IC50 = 0.969 mg mL-1 respectively). For A. nodosum and P. canaliculata, strongest inhibition occurred with ethanol pellet (IC50 = 0.238 and 0.228 mg mL−1, respectively). These inhibitory effects were validated in a model gut system. The data presented herein suggests the use of seaweed as a potential weight management tool is deserving of further investigation.