A pathology-MRI study of the short-T2 component in formalin-fixed multiple sclerosis brain
A pathology-MRI study of the short-T2 component in formalin-fixed multiple sclerosis brain
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DOI：
10.1212/wnl.55.10.1506
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发表时间：
2000-11-28
期刊：
影响因子：
9.9
通讯作者：
Paty, DW
中科院分区：
文献类型：
--
作者：
Moore, GRW;Leung, E;Paty, DW
目的：确定MS中T2弛豫分布中未显示短T2分量信号的区域的病理基础，如在福尔马林固定的脑中所研究的。工作背景：髓鞘特异性MRI信号在评估MS患者脱髓鞘中具有重要意义。证据表明，T2弛豫分布的短T2（10至50毫秒）成分源自髓鞘中的水。作者介绍了两例MS，其中短T2成分的解剖分布与死后福尔马林固定脑的病理结果相关。方法：将福尔马林固定的大脑的一半悬浮在用戊二醛交联的明胶-白蛋白混合物中，并用32回波MRI序列进行扫描。然后沿扫描的5-mm切片的中心沿着切割脑，拍照，脱水，并包埋在石蜡中。石蜡切片，用Luxol坚牢蓝和免疫细胞化学染色的2 '，3'-环核苷酸3 '-磷酸水解酶的髓鞘和通过Bielschowsky技术的轴突，与可比较的图像切片的短T2分量的振幅分布进行比较。结果：短T2信号的解剖分布与髓鞘分布相对应。伴有髓鞘丢失的慢性静止性MS斑块与缺乏短T2信号的区域相关。损伤内轴突的数量减少，但在髓鞘完全丧失的这些区域也看到许多存活的轴突。结论：在福尔马林固定的MS脑中，T2弛豫分布的短Ta分量对应于髓鞘的解剖分布。慢性、沉默的脱髓鞘MS斑块显示缺乏短Ta成分信号。这些结果支持了短T2组分起源于与髓鞘相关的水的假设。
Objective: To determine the pathologic basis of areas not exhibiting signal of the short-T2 component of the T2 relaxation distribution in MS, as studied in formalin-fixed brain. Background: A myelin-specific MRI signal would be of great importance in assessing demyelination in patients with MS. Evidence indicates that the short-T2 (10 to 50 millisecond) component of the T2 relaxation distribution originates from water in myelin sheaths. The authors present two cases of MS in which the anatomic distribution of the short-T2 component was correlated with the pathologic findings in postmortem formalin-fixed brain. Method: One half of the formalin-fixed brain was suspended in a gelatin-albumin mixture cross-linked with glutaraldehyde, and scanned with a 32-echo MRI sequence. The brain was then cut along the center of the 5-mm slices scanned, photographed, dehydrated, and embedded in paraffin. Paraffin sections, stained with Luxol fast blue and immunocytochemically for 2',3'-cyclic nucleotide 3'-phosphohydrolase for myelin and by the Bielschowsky technique for axone, were compared with the distribution of the amplitude of the short-T2 component of the comparable image slices. Results: The anatomic distribution of the short-T2 component signal corresponded to the myelin distribution. Chronic, silent MS plaques with myelin loss correlated with areas of absence of short-T2 signal. The numbers of axone within lesions were reduced, but many surviving axons were also seen in these areas of complete loss of myelin. Conclusion: In formalin-fixed MS brains the short-Ta component of the T2 relaxation distribution corresponds to the anatomic distribution of myelin. Chronic, silent demyelinated MS plaques show absence of the short-Ta component signal. These results support the hypothesis that the short-T2 component originates from water related to myelin.