Quantification of starch in plant tissues
Quantification of starch in plant tissues
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DOI：
10.1038/nprot.2006.232
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Zeeman, Samuel C.
中科院分区：
文献类型：
--
作者：
Smith, Alison M.;Zeeman, Samuel C.
本方案描述了一种简单的方法，通过溶解淀粉，将其定量转化为葡萄糖并测定葡萄糖来测量植物组织中的淀粉含量。植物组织最初必须迅速冷冻以停止新陈代谢，然后提取以去除游离葡萄糖。淀粉通过加热溶解，然后通过添加葡聚糖水解酶消化成葡萄糖。葡萄糖是用酶测定的。该方法比基于碘的方法更敏感和准确，并且适用于具有广泛淀粉含量的组织。多个样品的测量可在一天内完成。
This protocol describes a simple means of measuring the starch content of plant tissues by solubilizing the starch, converting it quantitatively to glucose and assaying the glucose. Plant tissue must initially be frozen rapidly to stop metabolism, then extracted to remove free glucose. Starch is solubilized by heating, then digested to glucose by adding glucan hydrolases. Glucose is assayed enzymatically. The method is more sensitive and accurate than iodine-based protocols, and is suitable for tissues that have a wide range of starch contents. Measurements on multiple samples can be completed within a day.