A Portable Array Biosensor for Detecting Multiple Analytes in Complex Samples
A Portable Array Biosensor for Detecting Multiple Analytes in Complex Samples
复制标题
用于检测复杂样品中多种分析物的便携式阵列生物传感器
作者：
C. Taitt;J. Golden;Y. Shubin;L. Shriver;K. Sapsford;A. Rasooly;F. Ligler
多分析物阵列生物传感器 (MAAB) 是由海军研究实验室 (NRL) 开发的，其目标是通过最少的用户操作同时检测和识别复杂样品中的多种目标物质。本文将重点介绍该仪器在生化和工程方面的最新改进。这些改进使得检测的分析物种类得以扩展，包括鼠伤寒沙门氏菌和单核细胞增生李斯特氏菌，并且还扩展了测试的不同样品基质。此外，生化测定的所有成分都可以在样品测试之前准备好，从而形成“即插即用”的方法。使用新型流体立方体模块演示了三种毒素（蓖麻毒素、葡萄球菌肠毒素 B 和霍乱毒素）的同时检测，该模块将操作次数限制为仅初始样品加载。这项工作展示了 MAAB 在快速分析具有多分析物功能的复杂样品方面的实用性，并且样品制备或最终分析所需的操作员操作最少。
The Multi-Analyte Array Biosensor (MAAB) has been developed at the Naval Research Laboratory (NRL) with the goal of simultaneously detecting and identifying multiple target agents in complex samples with minimal user manipulation. This paper will focus on recent improvements in the biochemical and engineering aspects of this instrument. These improvements have enabled the expansion of the repertoire of analytes detected to include Salmonella typhimurium and Listeria monocytogenes, and also expanded the different sample matrices tested. Furthermore, all components of the biochemical assays could be prepared well in advance of sample testing, resulting in a “plug-and-play” methodology. Simultaneous detection of three toxins (ricin, staphylococcal enterotoxin B, and cholera toxin) was demonstrated using a novel fluidics cube module that limits the number of manipulations to only the initial sample loading. This work demonstrates the utility of the MAAB for rapid analysis of complex samples with multianalyte capability, with a minimum of operator manipulations required for either sample preparation or final analysis.
与沙门氏菌病爆发相关的苜蓿种子发芽期间沙门氏菌的生长。
影响因子：
2
作者：
Stewart,DS;Reineke,KF;Ulaszek,JM;Tortorello,ML
通讯作者：
Tortorello,ML