Protective Effect of Lycium ruthenicum Murr. Against Radiation Injury in Mice.
Protective Effect of Lycium ruthenicum Murr. Against Radiation Injury in Mice.
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DOI：
10.3390/ijerph120708332
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发表时间：
2015-07-17
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
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作者：
Duan Y;Chen F;Yao X;Zhu J;Wang C;Zhang J;Li X
枸杞子穆尔的保护作用。抗辐射损伤的作用。昆明种小鼠随机分为对照组、模型组、阳性药物组和L。高剂量（8 g/kg），L.中剂量（4g/kg），L.在照射后第3、7、14天分别给予低剂量（2g/kg）的人参皂苷处理组。L.三个治疗组小鼠经口给予扁果提取物，对照组和模型组小鼠经口给予生理盐水，连续14天。阳性对照组于照射前30 min给予氨磷汀（WR-2721）。除对照组外，各组小鼠一次在其全身均匀地接受5戈伊量的X射线照射。分别于照射后第3、7、14天观察大鼠体重、血象、胸腺和脾脏指数、DNA、caspase-3、caspase-6和P53含量。L.扁果草能显著增加红细胞总数、血红蛋白计数和DNA含量（p < 0.05）。脾指数在照射后第3天和第14天显著恢复（P < 0.05）。L.黄芪低剂量组照射后第3、7、14天小鼠血清caspase-3、caspase-6水平明显降低，L.中剂量组照射后第7天血清caspase-6水平下降。L.黄芪可降低P53的表达。结果表明，L.对小鼠辐射损伤有保护作用。
The protective effect of Lycium ruthenicum Murr. against radiation injury was examined in mice. Kunming mice were randomly divided into a control group, model group, positive drug group and L. ruthenicum high dose (8 g/kg), L. ruthenicum middle dose (4 g/kg), L. ruthenicum low dose (2 g/kg) treatment groups, for which doses were administered the third day, seventh day and 14th day after irradiation. L. ruthenicum extract was administered orally to the mice in the three treatment groups and normal saline was administered orally to the mice in the control group and model group for 14 days. The positive group was treated with amifostine (WR-2721) at 30 min before irradiation. Except for the control group, the groups of mice received a 5 Gy quantity of X-radiation evenly over their whole body at one time. Body weight, hemogram, thymus and spleen index, DNA, caspase-3, caspase-6, and P53 contents were observed at the third day, seventh day, and 14th day after irradiation. L. ruthenicum could significantly increase the total red blood cell count, hemoglobin count and DNA contents (p < 0.05). The spleen index recovered significantly by the third day and 14th day after irradiation (p < 0.05). L. ruthenicum low dose group showed a significant reduction in caspase-3 and caspase-6 of serum in mice at the third day, seventh day, and 14th day after irradiation and L. ruthenicum middle dose group experienced a reduction in caspase-6 of serum in mice by the seventh day after irradiation. L. ruthenicum could decrease the expression of P53. The results showed that L. ruthenicum had protective effects against radiation injury in mice.