Exploring Mn-doped ZnS quantum dots for the room-temperature phosphorescence detection of enoxacin in biological fluids
Exploring Mn-doped ZnS quantum dots for the room-temperature phosphorescence detection of enoxacin in biological fluids
复制标题
探索用于生物体液中依诺沙星室温磷光检测的锰掺杂 ZnS 量子点
DOI：
10.1021/ac800100y
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发表时间：
2008-05-15
影响因子：
7.4
通讯作者：
Yan, Xiu-Ping
中科院分区：
文献类型：
--
作者：
He, Yu;Wang, He-Fang;Yan, Xiu-Ping
虽然大多数研究工作集中在基于量子点（QD）的荧光传感器的发展，很少有人注意到量子点的磷光性质及其潜在的磷光检测。本研究利用Mn掺杂ZnS量子点的磷光特性，建立了一种简便、快速、经济、灵敏、选择性检测生物体液中依诺沙星的室温磷光（RTP）方法。本文报道的基于Mn掺杂ZnS量子点的RTP方法不需要使用脱氧剂和其他诱导剂，并且允许检测生物流体中的依诺沙星而不受基质的自发荧光和散射光的干扰。Mn掺杂的ZnS量子点在生物体液、生物分子和其他种类的抗生素中的主要相关金属离子的存在下提供了优异的检测依诺沙星的选择性。淬灭的磷光发射由于在1.0 μ M的依诺沙星的加入是不受5000倍过量的Na+和10000倍过量的K+，Mg 2+，和Ca 2+。氨基酸如色氨酸、组氨酸和L-半胱氨酸在依诺沙星1000倍浓度下不影响依诺沙星的检测。葡萄糖在10000倍依诺沙星浓度下不影响检测。典型的联合给药（主要是其他类型的抗生素），如头孢替唑、头孢哌酮、苯唑西林和穿琥宁钾分别允许50、10、100和50倍过量，而不干扰依诺沙星的检测。对0.4 μ M依诺沙星进行11次重复测定，精密度为1.8%（RSD）。依诺沙星的检测限为58.6 nM。人尿液和血清样品中依诺沙星的加标回收率范围为94 - 104%。本文利用所开发的基于纳米掺杂ZnS量子点的RTP方法，对健康志愿者口服依诺沙星后尿液中依诺沙星的浓度进行了监测。研究结果表明，掺杂量子点在RTP检测中具有广阔的应用前景。
While most research works focus on the development of quantum dots (QDs)-based fluorescence sensors, much less attention is paid to the phosphorescence properties of QDs and their potential for phosphorescence detection. In this work, the phosphorescence property of Mn-doped ZnS QDs is explored to develop a novel room-temperature phosphorescence (RTP) method for the facile, rapid, cost-effective, sensitive, and selective detection of enoxacin in biological fluids. The Mn-doped ZnS QDs-based RTP method reported here does not need the use of deoxidants and other inducers and allows the detection of enoxacin in biological fluids without interference from autofluorescence and the scattering light of the matrix. The Mn-doped ZnS QDs offer excellent selectivity for detecting enoxacin in the presence of the main relevant metal ions in biological fluids, biomolecules, and other kinds of antibiotics. Quenching of the phosphorescence emission due to the addition of enoxacin at 1.0 mu M is unaffected by 5000-fold excesses of Na+ and 10000-fold excesses of K+, Mg2+, and Ca2+. Amino acids such as tryptophan, histidine, and L-cysteine at 1000-fold concentration of enoxacin do not affect the detection of enoxacin. Glucose does not affect the detection at 10000-fold concentration of enoxacin. Typical coadministers (mainly other types of antibiotics) such as ceftezole, cefoperazone, oxacillin, and kalii dehydrographolidi succinas are permitted at 50-, 10-, 100-, and 50-fold excesses, respectively, without interference with the detection of enoxacin. The precision for 11 replicate detections of 0.4 mu M enoxacin is 1.8% (RSD). The detection limit for enoxacin is 58.6 nM. The recovery of spiked enoxacin in human urine and serum samples ranges from 94 to 104%. The developed moni-doped ZnS QDs-based RTP method is employed to monitor the time-dependent concentration of enoxacin in urine from a healthy volunteer after the oral medication of enoxacin. The investigation provides evidence that doped QDs are promising for RTP detection in further applications.