Similarities of murine gamma interferon and the lymphokine that renders macrophages cytotoxic.
Similarities of murine gamma interferon and the lymphokine that renders macrophages cytotoxic.
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鼠γ干扰素和使巨噬细胞具有细胞毒性的淋巴因子的相似之处。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Schultz
中科院分区：
文献类型：
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作者：
W. J. Kleinschmidt;R. Schultz
用不溶性(琼脂糖结合)刀豆蛋白A处理小鼠脾淋巴样细胞，将精制的γ-干扰素(干扰素-γ)和巨噬细胞激活因子(MAF)放入培养液中。同样，MAF是由T细胞与其他干扰素-γ诱导剂共同产生的。MAF诱导静息(非细胞毒性)巨噬细胞成为杀瘤细胞。我们比较了MAF和INF-γ的各种物理化学性质，它们被干扰素-γ抗体中和，以及它们的诱导期。在这些参数中，抗病毒活性的诱导与巨噬细胞杀瘤活性的诱导进行了比较。所研究的所有参数都表明这两种淋巴因子在物理性质、分子特征和生物学活性方面非常相似，这种相似性表明这两种淋巴因子位于同一分子中。这一结论将包括，像干扰素-α和干扰素-β一样，干扰素-γ能够激活巨噬细胞，从而对肿瘤细胞产生细胞毒作用。
Murine spleen lymphoid cells treated with insoluble (Sepharose bound) concanavalin A elaborate Gamma (immune) interferon (IFN-gamma) and macrophage activation factor (MAF) into the culture medium. Similarly, MAF is produced by T cells with other IFN-gamma inducers. MAF induces resting (noncytotoxic) macrophages to become tumoricidal. We have compared various physicochemical properties of MAF and INF-gamma, their neutralization by antibody to IFN-gamma, and their induction period. In these parameters, induction of antiviral activity was compared to induction of macrophage tumoricidal activity. All of the parameters studied have given results that demonstrate close similarity in the physical properties, molecular characteristics, and biological reactivities of the two lymphokines, and this similarity suggests that these two lymphokines reside in the same molecule. This conclusion would include that IFN-gamma, like IFN-alpha and IFN-beta, is capable of activating macrophages to become cytotoxic toward tumor cells.