Three-dimensional cell culture to model epithelia in the female reproductive system.
Three-dimensional cell culture to model epithelia in the female reproductive system.
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DOI：
10.1177/1933719107310872
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发表时间：
2007-12-01
期刊：
影响因子：
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通讯作者：
Lelievre, Sophie A
中科院分区：
文献类型：
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作者：
Adissu, Hibret A;Asem, Elikplimi K;Lelievre, Sophie A
在体外，三维(3D)细胞培养产生有价值的模型，模拟3D组织的组织和功能，并增强对正常和病理情况下细胞/组织功能的理解。组织的功能取决于细胞和细胞外基质之间的相互作用；因此，有效的3D细胞培养依赖于适当的细胞外基质信号的使用。3D细胞培养的显著进展来自于对女性生殖系统器官(包括乳腺、子宫和卵巢)上皮细胞的研究。这些模型表明，在静息期复制正常的组织结构是进行适当的生理和病理研究的先决条件。作者的目标是解释在3D细胞培养中模拟正常上皮组织和功能的细节方面的重要性，例如基尖极性，并讨论有效的3D细胞培养模型如何在生殖生物学中产生有意义的应用。
In vitro 3-dimensional (3D) cell cultures produce valuable models that mimic 3D tissue organization and function and enhance the understanding of cell/tissue function under normal and pathological situations. Tissue function depends on the interactions between cells and the extracellular matrix; thus, effective 3D cell cultures rely on the use of appropriate extracellular matrix cues. Noticeable progress in 3D cell culture was obtained from studies with epithelial cells from organs of the female reproductive system including the mammary glands, the uterus, and the ovaries. These models show that replicating normal tissue organization in the resting phase is a prerequisite for appropriate physiological and pathological investigations. The authors' goals are to explain the importance of mimicking detailed aspects of normal epithelial organization and function, such as basoapical polarity, in 3D cell culture and to discuss how effective 3D cell culture models can lead to meaningful applications in reproductive biology.