Endotoxin administration stimulates cerebral catecholamine release in freely moving rats as assessed by microdialysis.
Endotoxin administration stimulates cerebral catecholamine release in freely moving rats as assessed by microdialysis.
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通过微透析评估，内毒素给药刺激自由活动大鼠的脑儿茶酚胺释放。
DOI：
10.1002/jnr.490400316
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Dunn,AJ
中科院分区：
文献类型：
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作者：
Lavicky,J;Dunn,AJ
在体内微透析被用来测量细胞外浓度的变化，儿茶酚胺和吲哚胺在自由活动的大鼠在响应管理的内毒素（脂多糖，LPS）。透析探针被立体定位地放置在内侧下丘脑或内侧前额叶皮质中。我们使用了重复测量设计，其中每只大鼠接受LPS或盐水，每名受试者在一周后重新接受另一种治疗。腹腔注射LPS（5 μ g）后，透析液中去甲肾上腺素（NE）、多巴胺（DA）及除去甲甲肾上腺素外的其他代谢物浓度均升高187%、119%。透析液中NE和DA浓度在第4或第5次（20 min）采集时显著升高，在2 h左右达到峰值，约4 h后恢复到基线水平。20%）。然而，100 μ g剂量的LPS使内侧前额叶皮质的NE和DA透析液浓度增加到与下丘脑中5 μ g剂量相当的程度，并且反应时间更长。无法可靠地测量5-羟色胺的透析液浓度，但其分解代谢物5-羟基吲哚乙酸（5-HIAA）的浓度在两个区域也升高。5-HIAA的峰值出现在4小时左右。用吲哚美辛（10 mg/kg ip）预处理大鼠完全防止了内侧前额叶皮质中100 μ g LPS引起的变化。这些结果支持了早期的神经化学数据，表明LPS刺激大脑中DA和NE的释放，并且可能还释放5-羟色胺。© 1995 Wiley利斯公司
In vivo microdialysis was used to measure changes in extracellular concentrations of catecholamines and indolamines in freely moving rats in response to administration of endotoxin (lipopolysaccharide, LPS). Dialysis probes were placed stereotaxically in either the medial hypothalamus or the medial prefrontal cortex. We used a repeated‐measures design in which each rat received LPS or saline, and each subject was retested with the other treatment one week later. With the dialysis probes in the medial hypothalamus, intraperitoneal (ip) administration of LPS (5 μg) increased dialysate concentrations of norepinephrine (NE, 187%), dopamine (DA, 119%), and all their measured catabolites, except normetanephrine. Dialysate concentrations of NE and DA were elevated significantly in the fourth or fifth (20 min) collection period with a peak response at around 2 hr. They returned to baseline by about 4 hr. When the dialysis probes were placed in the medial prefrontal cortex, the same dose of LPS also elevated dialysate concentrations of NE and DA, but the increases were much smaller (ca. 20%). However, a dose of 100 μg LPS increased dialysate concentrations of NE and DA from the medial prefrontal cortex to an extent comparable to that of the 5 μg dose in the hypothalamus, and the response was more prolonged. Dialysate concentrations of serotonin could not be measured reliably, but those of its catabolite, 5‐hydroxyindoleacetic acid (5‐HIAA), were also elevated in both regions. The peak of 5‐HIAA occurred at around 4 hr. Pretreatment of the rats with indomethacin (10 mg/kg ip) completely prevented the changes due to 100 μg LPS in the medial prefrontal cortex. These results support earlier neurochemical data suggesting that LPS stimulates the release of both DA and NE in the brain, and probably also release of serotonin. © 1995 Wiley‐Liss, Inc.