Orally Administered Thermostable N-Acyl Homoserine Lactonase from Bacillus sp Strain AI96 Attenuates Aeromonas hydrophila Infection in Zebrafish
Orally Administered Thermostable N-Acyl Homoserine Lactonase from Bacillus sp Strain AI96 Attenuates Aeromonas hydrophila Infection in Zebrafish
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DOI：
10.1128/aem.06139-11
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发表时间：
2012-03-01
影响因子：
4.4
通讯作者：
Yao, Bin
中科院分区：
文献类型：
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作者：
Cao, Yanan;He, Suxu;Yao, Bin
N-酰化高丝氨酸内酯(AHL)内酯酶能够降解参与细菌群体感应的信号分子，因此是控制细菌感染的一种新方法。这是一个与芽孢杆菌AHL内酯酶活性有关的基因。克隆了一株全长753bp的菌株AI96，并在大肠杆菌中进行了表达。推导出芽孢杆菌的氨基酸序列。AI96 AIIA(AIIA(AI96))与其他芽孢杆菌的同源性最高。AHL内酰胺酶(类似于80%的序列同源性)，因此被归类为金属-β-内酰胺酶超家族的成员。AIIA(AI96)在10℃至40℃的pH 8.0下保持接近100%的活性，在70℃pH 8.0下至少1小时非常稳定；在这些条件下没有发现其他芽孢杆菌AHL内酯酶是稳定的。AIIA(AI96)不被蛋白酶和鲤鱼肠液消化，具有广谱底物专一性。在鱼饲料中添加AIIA(AI96)可显著降低斑马鱼对嗜水气单胞菌的感染。这是口服AHL内酯酶有效控制嗜水气单胞菌的第一次报告。
N-Acylated homoserine lactone (AHL) lactonases are capable of degrading signal molecules involved in bacterial quorum sensing and therefore represent a new approach to control bacterial infection. Here a gene responsible for the AHL lactonase activity of Bacillus sp. strain AI96, 753 bp in length, was cloned and then expressed in Escherichia coli. The deduced amino acid sequence of Bacillus sp. AI96 AiiA (AiiA(AI96)) is most similar to those of other Bacillus sp. AHL lactonases (similar to 80% sequence identity) and was consequently categorized as a member of the metallo-beta-lactamase superfamily. AiiA(AI96) maintains similar to 100% of its activity at 10 degrees C to 40 degrees C at pH 8.0, and it is very stable at 70 degrees C at pH 8.0 for at least 1 h; no other Bacillus AHL lactonase has been found to be stable under these conditions. AiiA(AI96) resists digestion by proteases and carp intestinal juice, and it has broad-spectrum substrate specificity. The supplementation of AiiA(AI96) into fish feed by oral administration significantly attenuated Aeromonas hydrophila infection in zebrafish. This is the first report of the oral administration of an AHL lactonase for the efficient control of A. hydrophila.