Therapeutic effects of 15 Hz pulsed electromagnetic field on diabetic peripheral neuropathy in streptozotocin-treated rats.
Therapeutic effects of 15 Hz pulsed electromagnetic field on diabetic peripheral neuropathy in streptozotocin-treated rats.
复制标题
15 Hz 脉冲电磁场对链脲佐菌素治疗大鼠糖尿病周围神经病变的治疗作用
DOI：
10.1371/journal.pone.0061414
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Luo E
中科院分区：
文献类型：
--
作者：
Lei T;Jing D;Xie K;Jiang M;Li F;Cai J;Wu X;Tang C;Xu Q;Liu J;Guo W;Shen G;Luo E
尽管已有大量临床研究报道脉冲电磁场(PEMF)对糖尿病周围神经病变(DPN)患者具有神经保护作用，但其临床应用仍存在争议。本研究旨在探讨PEMF是否具有减轻链脲佐菌素(STZ)诱导的糖尿病大鼠周围神经病变症状的治疗潜力。成年雄性SD大鼠随机分为3组(每组8只)：非糖尿病对照组(Control)、糖尿病+15 HzPEMF暴露组(DM+PEMF)、糖尿病+假PEMF暴露组(DM)。用行为学方法观察STZ治疗大鼠DPN的症状和体征。同时对坐骨神经进行超微结构观察和血管内皮生长因子(VEGF)免疫组织化学研究。在为期7周的实验观察中，我们发现PEMF刺激不能改变STZ治疗的DPN大鼠的高血糖和体重减轻。然而，PEMF刺激减轻了STZ治疗的DPN大鼠的异常发展，表现为机械刺激和热刺激引起的后爪收缩阈值增加，坐骨神经脱髓鞘和轴突增大程度减轻，血管内皮生长因子免疫染色减弱。目前的研究表明，PEMF治疗可以防止在DPN动物模型中观察到的异常发展。提示PEMF对受损神经有直接的矫正作用，有望成为治疗DPN的一种有潜力的非侵入性治疗手段。
Although numerous clinical studies have reported that pulsed electromagnetic fields (PEMF) have a neuroprotective role in patients with diabetic peripheral neuropathy (DPN), the application of PEMF for clinic is still controversial. The present study was designed to investigate whether PEMF has therapeutic potential in relieving peripheral neuropathic symptoms in streptozotocin (STZ)-induced diabetic rats. Adult male Sprague–Dawley rats were randomly divided into three weight-matched groups (eight in each group): the non-diabetic control group (Control), diabetes mellitus with 15 Hz PEMF exposure group (DM+PEMF) which were subjected to daily 8-h PEMF exposure for 7 weeks and diabetes mellitus with sham PEMF exposure group (DM). Signs and symptoms of DPN in STZ-treated rats were investigated by using behavioral assays. Meanwhile, ultrastructural examination and immunohistochemical study for vascular endothelial growth factor (VEGF) of sciatic nerve were also performed. During a 7-week experimental observation, we found that PEMF stimulation did not alter hyperglycemia and weight loss in STZ-treated rats with DPN. However, PEMF stimulation attenuated the development of the abnormalities observed in STZ-treated rats with DPN, which were demonstrated by increased hind paw withdrawal threshold to mechanical and thermal stimuli, slighter demyelination and axon enlargement and less VEGF immunostaining of sciatic nerve compared to those of the DM group. The current study demonstrates that treatment with PEMF might prevent the development of abnormalities observed in animal models for DPN. It is suggested that PEMF might have direct corrective effects on injured nerves and would be a potentially promising non-invasive therapeutic tool for the treatment of DPN.