Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro
Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro
复制标题
DOI：
10.1016/j.biomaterials.2004.02.034
复制
发表时间：
2005-01-01
期刊：
影响因子：
14
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Murphy, WL;Hsiong, S;Mooney, DJ
多能细胞类型正在迅速成为各种组织工程方案的关键组成部分，间充质干细胞（MSC）正在成为骨组织再生的重要工具。虽然几个可溶性信号影响成骨分化的神经间质干细胞在体外的特点是，相对较少的基板信号的影响所知。本研究的目的是阐明骨样矿物质（BLM），这是至关重要的骨结合到骨科植入材料的过程中，在体外对人骨髓间充质干细胞的成骨分化的影响。通过表面水解和随后在改性的模拟体液中孵育，在聚（丙交酯-共-乙交酯）（PLG）基底上生长BLM膜（碳酸磷灰石，Ca/P = 1.55）。BLM膜表现出显着增加的纤连蛋白的吸附，并支持增强的人间充质干细胞（hMSCs）相对于PLG基板的增殖。在没有成骨补充剂的情况下，hMSCs在BLM或PLG上没有显示成骨标志物的高表达。在成骨补充剂的存在下，人骨髓间充质干细胞表现出更大的成骨标志物的表达PLG基板上比BLM基板上，通过碱性磷酸酶活性和骨钙素的生产。总之，这些数据支持的概念，基板信号显着影响MSC的生长和分化，突出了干细胞为基础的组织工程方案中的载体材料组合物的重要性。(C)2004 Elsevier Ltd.保留所有权利。
Multipotent cell types are rapidly becoming key components in a variety of tissue engineering schemes, and mesenchymal stem cells (MSCs) are emerging as an important tool in bone tissue regeneration. Although several soluble signals influencing osteogenic differentiation of NISCs in vitro are well-characterized, relatively little is known about the influence of substrate signals. This study was aimed at elucidating the effects of a bone-like mineral (BLM), which is vital in the process of bone bonding to orthopedic implant materials, on the osteogenic differentiation of human MSCs in vitro. Growth of a BLM film (carbonate apatite, Ca/P = 1.55) on poly(lactide-co-glycolide) (PLG) substrates was achieved via surface hydrolysis and subsequent incubation in a modified simulated body fluid. The BLM film demonstrated significantly increased adsorption of fibronectin, and supported enhanced proliferation of human mesenchymal stem cells (hMSCs) relative to PLG substrates. In the absence of osteogenic supplements hMSCs did not display a high expression of osteogenic markers on BLM or PLG. In the presence of osteogenic supplements hMSCs exhibited greater expression of osteogenic markers on PLG substrates than on BLM substrates, as measured by alkaline phosphatase activity and osteocalcin production. Taken together, these data support the concept that substrate signals significantly influence MSC growth and differentiation, highlighting the importance of carrier material composition in stem cell-based tissue engineering schemes. (C) 2004 Elsevier Ltd. All rights reserved.