COMPARISON OF THE AMINO-ACID-SEQUENCES OF 9 DIFFERENT SEROTYPES OF HEPATITIS-B SURFACE-ANTIGEN AND GENOMIC CLASSIFICATION OF THE CORRESPONDING HEPATITIS-B VIRUS-STRAINS
COMPARISON OF THE AMINO-ACID-SEQUENCES OF 9 DIFFERENT SEROTYPES OF HEPATITIS-B SURFACE-ANTIGEN AND GENOMIC CLASSIFICATION OF THE CORRESPONDING HEPATITIS-B VIRUS-STRAINS
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DOI：
10.1099/0022-1317-73-5-1201
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发表时间：
1992-05-01
影响因子：
3.8
通讯作者：
MAGNIUS, LO
中科院分区：
文献类型：
--
作者：
NORDER, H;HAMMAS, B;MAGNIUS, LO
应用聚合酶链反应（PCR）技术扩增了12株B肝炎病毒（HBV）9种不同血清型的表面抗原（HBsAg）基因，并进行了序列测定。这些代表了1975年在巴黎举行的HBsAg亚型国际研讨会上定义的8种HBV毒株，即P1至P8，以及adrq亚型。还对来自撒哈拉以南非洲的其他HBV毒株（一个ayw 4、一个adw 4和一个ayw 1）的S基因进行了测序。这12个新的S基因序列的关系，以前发表的20个进行了研究，通过构建系统发育树，证实了以前的分类为4组，指定为A至D，基于18个完整的HBV基因组。将我们测序的S基因与这些基因组群进行关联，非洲来源的ayw 1和P6（adw 2）都被归为A群，参考P1（越南来源的ayw 1）被归为B群，P5（ayr）、P8（adr）和adrq-都与C群相关，P2（ayw 2）和P3（ayw 3）都可以归为D群。有趣的是，w 4血清型病毒的S基因，即P4（ayw 4）和P7（adw 4 q-），与先前的组和彼此之间的差异为4%或更多，表明它们被分类为两个新的组，为此提出了命名E和F。在A组和B组中也发现了指定ayw的基因组;以前测序的指定ayw亚型的基因组都局限于D组。有迹象表明，W的亚决定簇的表位位于氨基酸位置125至127。因此，在位置125和127处，ayw 1、ayw 2和adw 2分别具有T和P残基，而M和T残基在ayw 3的相应位置处。ayw 4和adw 4在残基127处都具有L，并且除了P5之外，所有表达r的菌株在位置126处具有I而不是T残基。
The surface (S) genes of 12 hepatitis B viruses (HBVs) encoding nine different serotypes of hepatitis B surface antigen (HBsAg) were amplified by the polymerase chain reaction and sequenced. These represented the eight strains of HBV, P1 to P8, defined at an international workshop on HBsAg subtypes in Paris in 1975, and the adrq- subtype. The S genes from additional HBV strains, one ayw4, one adw4 and one ayw1, of sub-Saharan African origin, were also sequenced. The relationship of these 12 new S gene sequences to those of the 20 published previously was investigated by constructing a phylogenetic tree, which confirmed a previous classification into four groups, designated A to D, based on 18 complete HBV genomes. When relating our sequenced S genes to these genomic groups, ayw1 of African origin and P6 (adw2) were both allocated to group A, the reference P1 (ayw1 of Vietnamese origin) was allocated to group B, P5 (ayr), P8 (adr) and adrq- were all related to group C, and P2 (ayw2) and P3 (ayw3) could both be allocated to group D. Interestingly, the S genes of w4 serotype viruses, i.e. P4 (ayw4) and P7 (adw4q-), differed by 4 % or more from both previous groups and from each other, suggesting their classification into two new groups, for which the designations E and F are proposed. Genomes specifying ayw were also found in groups A and B; previously sequenced genomes specifying the ayw subtype have all been confined to group D. There were indications that the epitope for subdeterminants of w resided at amino acid positions 125 to 127. Thus, at positions 125 and 127, ayw1, ayw2 and adw2 had T and P residues, respectively, whereas M and T residues were at the corresponding positions of ayw3. Both ayw4 and adw4 had L at residue 127, and all strains expressing r, apart from P5, had an I instead of a T residue at position 126.