Abnormal mitosis induced by wheat-rye 1R monosomic addition lines
Abnormal mitosis induced by wheat-rye 1R monosomic addition lines
复制标题
小麦-黑麦 1R 单体加成系诱导的异常有丝分裂
DOI：
10.1139/gen-2013-0115
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发表时间：
2014-01-01
期刊：
影响因子：
3.1
通讯作者：
Tang, Zong-Xiang
中科院分区：
文献类型：
--
作者：
Fu, Shu-Lan;Yang, Man-Yu;Tang, Zong-Xiang
八倍体小黑麦以普通小麦（Triticum aestivum L. '绵阳11'）×黑麦（Secalealeceae L. 'Kustro'）为原料，与'绵阳11'回交并自花受精获得部分后代。使用黑麦基因组 DNA 和重复序列 pAs1 和 pSc119.2 作为探针进行原位杂交来分析这些后代的有丝分裂染色体。鉴定出三个小麦-黑麦 1R 单体附加系和一个含有 1R 和 1BL.1RS 易位染色体的小麦系 (12FT-1685)。在两条系中观察到异常有丝分裂。在 1R 单体附加系 (3-8-20-1R-2) 有丝分裂期间，观察到滞后染色体、微核、染色体桥和 1R 染色体的一极分离。在品系12FT-1685和3-8-20-1R-2的一些自交后代植物中也观察到染色体的异常有丝分裂行为。这些后代含有 1R 染色体或 1R 染色体臂。此外，3-8-20-1R-2 的后代之一不存在 4B 染色体。在另外两个 1R 单体附加系中没有观察到染色体的这种异常有丝分裂行为。这些结果表明，添加到小麦中的单个1R染色体可能会导致小麦和黑麦染色体的异常有丝分裂行为，并且兄弟1R单体添加系之间可能会发生不同的遗传变异。
Octoploid triticale were derived from common wheat (Triticum aestivum L. 'Mianyang11') x rye (Secale cereale L. 'Kustro'), and some progeny were obtained by the backcrossing of triticale with 'Mianyang11' followed by self-fertilization. In situ hybridization using rye genomic DNA and repetitive sequences pAs1 and pSc119.2 as probes was used to analyze the mitotic chromosomes of these progeny. Three wheat-rye 1R monosomic addition lines and a wheat line (12FT-1685) containing a 1R and a 1BL.1RS translocation chromosome were identified. Abnormal mitosis was observed in the two lines. During mitosis of a 1R monosomic addition line (3-8-20-1R-2), lagging chromosomes, micronuclei, chromosomal bridges, and the one pole segregation of 1R chromosome were observed. Abnormal mitotic behaviour of chromosomes was also observed in some of the self-progeny plants of lines 12FT-1685 and 3-8-20-1R-2. These progeny contained 1R chromosome or 1R chromosome arm. In addition, 4B chromosomes were absent from one of the progeny of 3-8-20-1R-2. This abnormal mitotic behaviour of chromosomes was not observed in two other 1R monosomic addition lines. These results indicate that a single 1R chromosome added to wheat might cause abnormal mitotic behaviour of both wheat and rye chromosomes and different genetic variations might occurr among the sibling 1R monosomic addition lines.