Multipotent neural stem cells reside into the rostral extension and olfactory bulb of adult rodents
Multipotent neural stem cells reside into the rostral extension and olfactory bulb of adult rodents
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DOI：
10.1523/jneurosci.22-02-00437.2002
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发表时间：
2002-01-15
影响因子：
5.3
通讯作者：
Vescovi, AL
中科院分区：
文献类型：
--
作者：
Gritti, A;Bonfanti, L;Vescovi, AL
前脑侧脑室的侧壁是成年哺乳动物脑中干细胞最丰富的来源。这些干细胞产生新的嗅觉神经元，这些神经元在整个生命过程中不断更新。神经元起源于脑室下区（SVZ），在SVZ的吻侧延伸（RE）内沿着由胶质细胞形成的管状结构内的吻侧迁移流（RMS）迁移，最终到达嗅球（OB）。我们证明，与目前的观点相反，具有干细胞特征的多能（神经元-星形胶质细胞-少突胶质细胞）前体不仅可以从SVZ分离，而且可以从整个RE分离，包括OB内的远端部分。具体地说，这些干细胞不是来自SVZ的迁移性神经母细胞。有趣的是，从近端RE分离的干细胞产生显著更多的少突胶质细胞，而从远端RE分离的干细胞比从SVZ和其他RE区域衍生的干细胞增殖显著更慢。这些发现表明，干细胞并不局限于前脑室周区，并表明干细胞赋予不同的功能特性发生在不同水平的SVZ-RE途径。
The lateral walls of the forebrain lateral ventricles are the richest source of stem cells in the adult mammalian brain. These stem cells give rise to new olfactory neurons that are renewed throughout life. The neurons originate in the subventricular zone (SVZ), migrate within the rostral extension (RE) of the SVZ along the rostral migratory stream (RMS) within tube-like structures formed of glial cells, to eventually reach the olfactory bulb (OB). We demonstrate that, contrary to the current view, multipotential (neuronal-astroglial-oligodendroglial) precursors with stem cell features can be isolated not only from the SVZ but also from the entire RE, including the distal portion within the OB. Specifically, these stem cells do not derive from the migratory neuroblasts coming from the SVZ. Interestingly, stem cells isolated from the proximal RE generate significantly more oligodendrocytes, and those from the distal RE proliferate significantly more slowly than stem cells derived from the SVZ and other RE regions. These findings demonstrate that stem cells are not confined to the forebrain periventricular region and indicate that stem cells endowed with different functional characteristics occur at different levels of the SVZ-RE pathway.