ALYREF links 3-end processing to nuclear export of non-polyadenylated mRNAs
ALYREF links 3-end processing to nuclear export of non-polyadenylated mRNAs
复制标题
ALYREF 将 3 末端加工与非聚腺苷酸化 mRNA 的核输出联系起来
DOI：
10.15252/embj.201899910
复制
发表时间：
2019-05-02
期刊：
影响因子：
11.4
通讯作者：
Cheng, Hong
中科院分区：
文献类型：
--
作者：
Fan, Jing;Wang, Ke;Cheng, Hong
RNA结合蛋白TRIPREF在核输出和多聚腺苷酸化mRNA的3端加工中起关键作用，但这种调节是否也延伸到非多聚腺苷酸化RNA尚不清楚。复制依赖性（RD）组蛋白mRNA不是聚腺苷酸化的，而是以茎环（SL）结构结束。在这里，我们证明，RESREF蛋白结合的区域旁边的RD-组蛋白mRNA的SL。SL结合蛋白（SLBP）直接与BMPREF相互作用并促进其募集。ESPRREF通过与U 7-snRNP特异性组分Lsm 11的物理相互作用促进U 7-snRNP募集，从而促进组蛋白前mRNA 3-末端加工。此外，TREXREF与TREX复合物的其他组分一起增强组蛋白mRNA的输出。此外，我们发现，3端加工促进了BREF的招募和组蛋白mRNA的输出。总之，我们的结果表明ALYREF在协调非多聚腺苷酸化mRNA的3端加工和核输出方面发挥着重要作用。
The RNA-binding protein ALYREF plays key roles in nuclear export and also 3-end processing of polyadenylated mRNAs, but whether such regulation also extends to non-polyadenylated RNAs is unknown. Replication-dependent (RD)-histone mRNAs are not polyadenylated, but instead end in a stem-loop (SL) structure. Here, we demonstrate that ALYREF prevalently binds a region next to the SL on RD-histone mRNAs. SL-binding protein (SLBP) directly interacts with ALYREF and promotes its recruitment. ALYREF promotes histone pre-mRNA 3-end processing by facilitating U7-snRNP recruitment through physical interaction with the U7-snRNP-specific component Lsm11. Furthermore, ALYREF, together with other components of the TREX complex, enhances histone mRNA export. Moreover, we show that 3-end processing promotes ALYREF recruitment and histone mRNA export. Together, our results point to an important role of ALYREF in coordinating 3-end processing and nuclear export of non-polyadenylated mRNAs.