Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase mRNA levels in rat liver.
Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase mRNA levels in rat liver.
复制标题
大鼠肝脏中 3-羟基-3-甲基戊二酰辅酶 A 还原酶 mRNA 水平的调节。
DOI：
--
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
A. Fogelman
中科院分区：
文献类型：
--
作者：
C. Clarke;P. Edwards;S. Lan;R. Tanaka;A. Fogelman
据报道，在饲料中添加考来烯胺或考来烯胺加美维诺林可增加大鼠肝脏3-羟基-3-甲基戊二酰-CoA还原酶的活性和质量。目前的数据表明，这些相同的饮食操作引起功能性还原酶mRNA的诱导。从大鼠肝脏中分离RNA，并加入到体外翻译系统中，还原酶进行免疫沉淀，并在变性条件下通过聚丙烯酰胺凝胶电泳进行分析。一种蛋白质被特异性免疫沉淀，并发现在0.5 M尿素/十二烷基硫酸钠/聚丙烯酰胺凝胶上的Mr为90，000，在8 M尿素/十二烷基硫酸钠/聚丙烯酰胺凝胶上的Mr为94，000。在喂食补充有5%考来烯胺和0.1%美维诺林的大鼠饲料的动物中，还原酶mRNA水平比喂食5%考来烯胺的大鼠饲料的动物高5.7倍，比喂食5%考来烯胺的大鼠饲料并通过胃插管给予甲羟戊酸内酯的动物高16倍。从饲喂正常饮食并在酶活性昼夜循环的最低点杀死的动物中分离的RNA不含可检测量的还原酶mRNA，如通过该测定所确定的。
Addition of cholestyramine or cholestyramine plus mevinolin to the diet has been reported to increase the activity and mass of rat liver 3-hydroxy-3-methylglutaryl-CoA reductase. The present data show that these same dietary manipulations cause an induction of functional reductase mRNA. RNA was isolated from rat livers and added to an in vitro translation system, and the reductase was immunoprecipitated and analyzed by polyacrylamide gel electrophoresis under denaturing conditions. One protein was specifically immunoprecipitated and was found to have a Mr of 90,000 on 0.5 M urea/sodium dodecyl sulfate/polyacrylamide gels and a Mr of 94,000 on 8 M urea/sodium dodecyl sulfate/polyacrylamide gels. In animals fed rat chow supplemented with 5% cholestyramine and 0.1% mevinolin, reductase mRNA levels were 5.7-fold higher than in animals fed rat chow with 5% cholestyramine and were 16-fold higher than in animals fed rat chow with 5% cholestyramine and given mevalonolactone by stomach intubation. RNA isolated from animals fed a normal diet and killed at the nadir of the diurnal cycle of enzyme activity contained no detectable amounts of reductase mRNA as determined by this assay.