Discrimination of bovine milk from non-dairy milk by lipids fingerprinting using routine matrix-assisted laser desorption ionization mass spectrometry
Discrimination of bovine milk from non-dairy milk by lipids fingerprinting using routine matrix-assisted laser desorption ionization mass spectrometry
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DOI：
10.1038/s41598-020-62113-9
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发表时间：
2020-03-20
影响因子：
4.6
通讯作者：
Larrouy-Maumus, Gerald
中科院分区：
文献类型：
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作者：
England, Philippa;Tang, Wenhao;Larrouy-Maumus, Gerald
任何国家的一个重要可持续发展目标都是通过生产充足和安全的粮食供应来确保粮食安全。这是牛乳的情况，其中添加非乳制品牛奶，如蔬菜（例如，大豆或椰子）已成为掺假和欺诈的常见来源。通常，气相色谱技术用于检测关键脂类（例如，三酰基甘油），具有评估牛奶来源的有效读数和检测牛牛奶中的外来牛奶。然而，这种方法需要几个样品制备步骤和专用的实验室环境，妨碍了高通量过程。为了满足这一需求，本研究旨在建立一种新的、简便的、无需有机溶剂提取的基于脂质指纹图谱的常规MALDI-TOF质谱法（MS）检测牛乳和非乳制品的方法。优化的方法是将牛奶在水中简单稀释，然后在正线性离子模式下进行MALDI-TOF质谱分析，基质为2,5-二羟基苯甲酸和2-羟基-5甲氧基苯甲酸（super-DHB）以10 mg/mL溶于70%乙醇的比例为9:1的混合物。这种灵敏、廉价、快速的方法在食品真实性应用中具有潜在的用途。
An important sustainable development goal for any country is to ensure food security by producing a sufficient and safe food supply. This is the case for bovine milk where addition of non-dairy milks such as vegetables (e.g., soya or coconut) has become a common source of adulteration and fraud. Conventionally, gas chromatography techniques are used to detect key lipids (e.g., triacylglycerols) has an effective read-out of assessing milks origins and to detect foreign milks in bovine milks. However, such approach requires several sample preparation steps and a dedicated laboratory environment, precluding a high throughput process. To cope with this need, here, we aimed to develop a novel and simple method without organic solvent extractions for the detection of bovine and non-dairy milks based on lipids fingerprint by routine MALDI-TOF mass spectrometry (MS). The optimized method relies on the simple dilution of milks in water followed by MALDI-TOF MS analyses in the positive linear ion mode and using a matrix consisting of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5methoxybenzoic acid (super-DHB) solubilized at 10 mg/mL in 70% ethanol. This sensitive, inexpensive, and rapid method has potential for use in food authenticity applications.