Determination of sorbitol and galactitol at the nanogram level in biological samples by high-performance liquid chromatography.
Determination of sorbitol and galactitol at the nanogram level in biological samples by high-performance liquid chromatography.
复制标题
采用高效液相色谱法测定生物样品中纳克级的山梨醇和半乳糖醇。
DOI：
10.1016/0003-2697(84)90565-7
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发表时间：
1984
影响因子：
2.9
通讯作者：
A. Lenaers
中科院分区：
文献类型：
--
作者：
J. Dethy;B. Callaert;M. Janssens;A. Lenaers
醛糖还原酶是一种在糖尿病并发症如白内障和神经病变中起重要作用的酶。估计这种酶在体内的酶活性的最佳方法包括测量山梨醇或半乳糖醇在各种类型的细胞或组织中的积累。建立了生物样品中多元醇的高效液相色谱测定方法。该方法基于这样的事实，即多元醇如山梨糖醇和半乳糖醇与异氰酸苯酯反应，产生在240 nm处吸收紫外线的衍生物。应用程序的分离和测定多元醇的生物样品的各种来源，如晶状体，坐骨神经，人皮肤成纤维细胞，红细胞，进行了说明和说明。
Aldose reductase is an enzyme that plays an important role in diabetic complications such as cataract and neuropathy. The best way to estimate the enzymatic activity of this enzyme in vivo consists of measuring the accumulation of sorbitol or galactitol in various types of cells or tissues. A sensitive method to measure the polyols in biological samples by high-performance liquid chromatography has been developed. This method is based on the fact that polyols like sorbitol and galactitol react with phenylisocyanate to yield uv-absorbing derivatives at 240 nm. Applications to the separation and determination of polyols in biological samples of various origins, such as lenses, sciatic nerves, human skin fibroblasts, and red cells, are described and illustrated.